Absolute quantification of targeted rabbit liver- and meat tissue-specific peptide markers in highly processed food products.

Autor: Stachniuk A; Department of Bioanalytics, Medical University of Lublin, ul. Jaczewskiego 8b, 20-090 Lublin, Poland. Electronic address: anna.stachniuk@umlub.pl., Trzpil A; Department of Bioanalytics, Medical University of Lublin, ul. Jaczewskiego 8b, 20-090 Lublin, Poland., Czeczko R; Department of Chemistry, University of Live Sciences in Lublin, ul. Akademicka 15, 20-950 Lublin, Poland., Nowicki Ł; Altium International Sp. z o.o, ul. Puławska 303, 02-785 Warszawa, Poland., Ziomkowska M; Altium International Sp. z o.o, ul. Puławska 303, 02-785 Warszawa, Poland., Fornal E; Department of Bioanalytics, Medical University of Lublin, ul. Jaczewskiego 8b, 20-090 Lublin, Poland.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Apr 16; Vol. 438, pp. 138069. Date of Electronic Publication: 2023 Nov 23.
DOI: 10.1016/j.foodchem.2023.138069
Abstrakt: A highly sensitive and selective method for the simultaneous absolute quantification of peptides unique to rabbit meat- and liver-specific tissue was developed using liquid chromatography - triple quadrupole mass spectrometry. Two rabbit skeletal muscle-specific peptides (SSVFVADPK and PHSHPALTPEQK), three rabbit liver tissue-specific peptides (FNLEALVTHTLPFEK, AILNYVANK, and TELAEPTSTR) and one peptide specific to both rabbit offal and skeletal muscle tissue (AFFGHYLYEVAR) were monitored. Analyses were performed using peptides labelled with stable isotopes ( 13 C and 15 N) as internal standards. Fifteen food samples containing rabbit meat and/or liver were analysed to verify compliance of the rabbit meat and liver composition with product labelling. One sample was adulterated with undeclared rabbit liver. The limit of detection and limit of quantification for the selected peptides of interest were in the range of 0.17 to 0.35 ng/mg and 0.57 to 1.17 ng/mg, respectively. The method may be useful for the determination of rabbit meat and liver tissue in highly processed food samples.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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