eDNA monitoring as a tool for evaluating the reintroduction of Austropotamobius pallipes after a crayfish plague outbreak.

Autor: Casabella-Herrero G; Mycology Department, Real Jardín Botánico-CSIC, Plaza Murillo 2, 28014 Madrid, Spain. Electronic address: gcasabella@rjb.csic.es., Martín-Torrijos L; Mycology Department, Real Jardín Botánico-CSIC, Plaza Murillo 2, 28014 Madrid, Spain. Electronic address: lmtorrijos@rjb.csic.es., Diéguez-Uribeondo J; Mycology Department, Real Jardín Botánico-CSIC, Plaza Murillo 2, 28014 Madrid, Spain. Electronic address: dieguez@rjb.csic.es.
Jazyk: angličtina
Zdroj: Journal of invertebrate pathology [J Invertebr Pathol] 2023 Nov; Vol. 201, pp. 108026. Date of Electronic Publication: 2023 Nov 24.
DOI: 10.1016/j.jip.2023.108026
Abstrakt: The crayfish plague, a severe disease caused by the oomycete Aphanomyces astaci, is responsible for most population declines of susceptible crayfish in Europe. This pathogen has been devastating native populations of Austropotamobius pallipes since the 1970s in the Iberian Peninsula. In this study, we report a massive mortality event in one of the most important Spanish populations of A. pallipes. We aimed to: (i) identify the cause of the mortality, and (ii) evaluate the reintroduction viability of the species. Over the course of six months, we used environmental DNA (eDNA) and traditional trap-based methods to detect the presence of A. astaci or of native or invasive crayfish in order to evaluate the reintroduction viability of A. pallipes to the affected population. We did not capture any live crayfish or detect the presence of A. astaci in the reservoir water during the six months following the mass mortality event. Our analyses indicated that it was feasible to initiate a reintroduction program at the site, which will continue to be monitored for three to five years and will help improve the conservation status of A. pallipes.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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