An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection.

Autor:	Skakic I; School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia., Francis JE; School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia., Dekiwadia C; RMIT Microscopy and Microanalysis Facility, School of Science, RMIT University, Melbourne, VIC 3001, Australia., Aibinu I; School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia.; Department of Health, Science and Community, Swinburne University of Technology, Hawthorn, VIC 3122, Australia., Huq M; School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia.; Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia., Taki AC; Faculty of Science, Melbourne Veterinary School, The University of Melbourne, Parkville, VIC 3010, Australia., Walduck A; School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia.; Rural Health Research Institute, Charles Sturt University, Orange, NSW 2800, Australia., Smooker PM; School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia.
Jazyk:	angličtina
Zdroj:	Vaccines [Vaccines (Basel)] 2023 Oct 27; Vol. 11 (11). Date of Electronic Publication: 2023 Oct 27.
DOI:	10.3390/vaccines11111652
Abstrakt:	Using removable silica templates, protein nanocapsules comprising the A subunit of Helicobacter pylori urease (UreA) were synthesised. The templates were of two sizes, with solid core mesoporous shell (SC/MS) silica templates giving rise to nanocapsules of average diameter 510 nm and mesoporous (MS) silica templates giving rise to nanocapsules of average diameter 47 nm. Both were shown to be highly monodispersed and relatively homogenous in structure. Various combinations of the nanocapsules in formulation were assessed as vaccines in a mouse model of H. pylori infection. Immune responses were evaluated and protective efficacy assessed. It was demonstrated that vaccination of mice with the larger nanocapsules combined with an adjuvant was able to significantly reduce colonisation. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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