| Autor: |
Melouli H; Viral Oncogenesis Laboratory, Pasteur Institute of Algeria, Algiers 16000, Algeria., Khenchouche A; Laboratory of Applied Biochemistry, Ferhat Abbas, Setif 1 University, Setif 19000, Algeria., Taibi-Zidouni F; Viral Oncogenesis Laboratory, Pasteur Institute of Algeria, Algiers 16000, Algeria., Salma D; Viral Oncogenesis Laboratory, Pasteur Institute of Algeria, Algiers 16000, Algeria., Aoudia N; Viral Oncogenesis Laboratory, Pasteur Institute of Algeria, Algiers 16000, Algeria., Djennaoui D; Otorhinolaryngology Department, Mustapha Pacha Hospital, Algiers 16000, Algeria., Sahraoui T; Laboratory of Developmental Biology and Differentiation, Es-Sénia University, Oran 31000, Algeria., Benyahia S; Otorhinolaryngology Department, Mustapha Pacha Hospital, Algiers 16000, Algeria., El Kebir FZ; Laboratory of Developmental Biology and Differentiation, Es-Sénia University, Oran 31000, Algeria. |
| Abstrakt: |
Nasopharyngeal cancer (NPC) is a prevalent type of cancer that often takes the form of undifferentiated carcinoma in the Maghreb region. It affects people of all ages. NPC diagnosis, mainly based on detecting Epstein-Barr virus (EBV), has not been well evaluated in North Africa. We compared the classical EBV serological tests using indirect immunofluorescence to the detection of EBV DNase antibodies by immunoblot in Algerian NPC patients. Significant variations were observed among different age groups of patients regarding the presence of VCA-IgA antibodies (0-14 and ≥30 years old, p < 0.0001; 15-19 and ≥30 years old, p < 0.01) and EA-IgA (0-14 and ≥30 years old, p < 0.01; 15-29 and ≥30 years old, p < 0.05). Differences were also noted in the titers of IgA anti-VCA and anti-EA antibodies across the three age groups. Some patients under the age of 30 with detectable IgG anti-VCA antibodies had undetectable IgA anti-VCA antibodies. These patients had a strong anti-DNase IgA response. However, older individuals had a higher level of anti-DNase IgG. Before treatment, children had strong DNase reactivity as indicated by specific IgA antibodies. Young adults had high IgA anti-DNase response, but the elderly (90.9%) had a lower response for these antibodies. Following therapy, the children retained high levels of IgA anti-DNase antibodies, and 66% of the young adults demonstrated robust antibody reactivity against DNase. In contrast, IgG responses to anti-DNase were low in children. This study demonstrated the utility of anti-DNase responses in the diagnosis and prognosis of NPC. |
