Expanding the Potential of Self-Assembled Silk Fibroin as Aerogel Particles for Tissue Regeneration.

Autor: Bernardes BG; Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.; AerogelsLab, I+D Farma Group (GI-1645), Department of Pharmacology, Pharmacy and Pharmaceutical Technology, iMATUS and Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain., Baptista-Silva S; Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal., Illanes-Bordomás C; AerogelsLab, I+D Farma Group (GI-1645), Department of Pharmacology, Pharmacy and Pharmaceutical Technology, iMATUS and Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain., Magalhães R; Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal., Dias JR; Centre for Rapid and Sustainable Product Development, Instituto Politécnico de Leiria, 2430-028 Marinha Grande, Portugal., Alves NMF; Centre for Rapid and Sustainable Product Development, Instituto Politécnico de Leiria, 2430-028 Marinha Grande, Portugal., Costa R; Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.; Biochemistry Unit, Department of Biomedicine, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal., García-González CA; AerogelsLab, I+D Farma Group (GI-1645), Department of Pharmacology, Pharmacy and Pharmaceutical Technology, iMATUS and Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain., Oliveira AL; Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
Jazyk: angličtina
Zdroj: Pharmaceutics [Pharmaceutics] 2023 Nov 09; Vol. 15 (11). Date of Electronic Publication: 2023 Nov 09.
DOI: 10.3390/pharmaceutics15112605
Abstrakt: A newly produced silk fibroin (SF) aerogel particulate system using a supercritical carbon dioxide (scCO 2 )-assisted drying technology is herein proposed for biomedical applications. Different concentrations of silk fibroin (3%, 5%, and 7% ( w / v )) were explored to investigate the potential of this technology to produce size- and porosity-controlled particles. Laser diffraction, helium pycnometry, nitrogen adsorption-desorption analysis and Fourier Transform Infrared with Attenuated Total Reflectance (FTIR-ATR) spectroscopy were performed to characterize the physicochemical properties of the material. The enzymatic degradation profile of the SF aerogel particles was evaluated by immersion in protease XIV solution, and the biological properties by cell viability and cell proliferation assays. The obtained aerogel particles were mesoporous with high and concentration dependent specific surface area (203-326 m 2 /g). They displayed significant antioxidant activity and sustained degradation in the presence of protease XIV enzyme. The in vitro assessment using human dermal fibroblasts (HDF) confirm the particles' biocompatibility, as well as the enhancement in cell viability and proliferation.
Databáze: MEDLINE
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