Neutrophil as a Carrier for Cancer Nanotherapeutics: A Comparative Study of Liposome, PLGA, and Magnetic Nanoparticles Delivery to Tumors.

Autor: Garanina AS; Laboratory of Biomedical Nanomaterials, National University of Science and Technology «MISIS», 119049 Moscow, Russia., Vishnevskiy DA; Department of Medical Nanobiotechnology, N.I. Pirogov Russian National Research Medical University, 117997 Moscow, Russia.; V. Serbsky National Medical Research Center for Psychiatry and Narcology, 119034 Moscow, Russia., Chernysheva AA; V. Serbsky National Medical Research Center for Psychiatry and Narcology, 119034 Moscow, Russia., Valikhov MP; Department of Medical Nanobiotechnology, N.I. Pirogov Russian National Research Medical University, 117997 Moscow, Russia.; V. Serbsky National Medical Research Center for Psychiatry and Narcology, 119034 Moscow, Russia., Malinovskaya JA; D. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia., Lazareva PA; Department of Medical Nanobiotechnology, N.I. Pirogov Russian National Research Medical University, 117997 Moscow, Russia., Semkina AS; Department of Medical Nanobiotechnology, N.I. Pirogov Russian National Research Medical University, 117997 Moscow, Russia.; V. Serbsky National Medical Research Center for Psychiatry and Narcology, 119034 Moscow, Russia., Abakumov MA; Laboratory of Biomedical Nanomaterials, National University of Science and Technology «MISIS», 119049 Moscow, Russia.; Department of Medical Nanobiotechnology, N.I. Pirogov Russian National Research Medical University, 117997 Moscow, Russia., Naumenko VA; V. Serbsky National Medical Research Center for Psychiatry and Narcology, 119034 Moscow, Russia.
Jazyk: angličtina
Zdroj: Pharmaceuticals (Basel, Switzerland) [Pharmaceuticals (Basel)] 2023 Nov 06; Vol. 16 (11). Date of Electronic Publication: 2023 Nov 06.
DOI: 10.3390/ph16111564
Abstrakt: Insufficient drug accumulation in tumors is still a major concern for using cancer nanotherapeutics. Here, the neutrophil-based delivery of three nanoparticle types-liposomes, PLGA, and magnetite nanoparticles-was assessed both in vitro and in vivo. Confocal microscopy and a flow cytometry analysis demonstrated that all the studied nanoparticles interacted with neutrophils from the peripheral blood of mice with 4T1 mammary adenocarcinoma without a significant impact on neutrophil viability or activation state. Intravital microscopy of the tumor microenvironment showed that the neutrophils did not engulf the liposomes after intravenous administration, but facilitated nanoparticle extravasation in tumors through micro- and macroleakages. PLGA accumulated along the vessel walls in the form of local clusters. Later, PLGA nanoparticle-loaded neutrophils were found to cross the vascular barrier and migrate towards the tumor core. The magnetite nanoparticles extravasated in tumors both via spontaneous macroleakages and on neutrophils. Overall, the specific type of nanoparticles largely determined their behavior in blood vessels and their neutrophil-mediated delivery to the tumor. Since neutrophils are the first to migrate to the site of inflammation, they can increase nanodrug delivery effectiveness for nanomedicine application.
Databáze: MEDLINE
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