| Autor: |
Andrei C; Faculty of Pharmacy, 'Carol Davila' University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania., Mihai DP; Faculty of Pharmacy, 'Carol Davila' University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania., Nitulescu G; Faculty of Pharmacy, 'Carol Davila' University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania., Ungurianu A; Faculty of Pharmacy, 'Carol Davila' University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania., Margina DM; Faculty of Pharmacy, 'Carol Davila' University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania., Nitulescu GM; Faculty of Pharmacy, 'Carol Davila' University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania., Olaru OT; Faculty of Pharmacy, 'Carol Davila' University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania., Busca RM; Colentina Clinical Hospital, Stefan cel Mare 19-21, 020125 Bucharest, Romania., Zanfirescu A; Faculty of Pharmacy, 'Carol Davila' University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania. |
| Abstrakt: |
Osteoarthritis is characterized by progressive articular cartilage degradation, subchondral bone changes, and synovial inflammation, and affects various joints, causing pain and disability. Current osteoarthritis therapies, primarily focused on pain management, face limitations due to limited effectiveness and high risks of adverse effects. Safer and more effective treatments are urgently needed. Considering that the endocannabinoid 2-arachidonoyl glycerol is involved in pain processing, increasing its concentration through monoacylglycerol lipase (MAGL) inhibition reduces pain in various animal models. Furthermore, drug repurposing approaches leverage established drug safety profiles, presenting a cost-effective route to accelerate clinical application. To this end, cetirizine and levetiracetam were examined for their MAGL inhibitory effects. In vitro studies revealed that cetirizine and levetiracetam inhibited MAGL with IC 50 values of 9.3931 µM and 3.0095 µM, respectively. In vivo experiments demonstrated that cetirizine, and to a lesser extent levetiracetam, reduced mechanical and thermal nociception in complete Freund adjuvant (CFA)-induced osteoarthritis in rats. Cetirizine exhibited a notable anti-inflammatory effect, reducing CFA-induced inflammation, as well as the inflammatory infiltrate and granuloma formation in the affected paw. These findings suggest that cetirizine may serve as a promising starting point for the development of novel compounds for osteoarthritis treatment, addressing both pain and inflammation. |
