| Autor: |
Ilosvai ÁM; Institute of Chemistry, University of Miskolc, 3515 Miskolc, Hungary.; Higher Education and Industrial Cooperation Centre, University of Miskolc, 3515 Miskolc, Hungary., Forgách L; Department of Biophysics and Radiation Biology, Semmelweis University, 1094 Budapest, Hungary., Kovács N; Department of Biophysics and Radiation Biology, Semmelweis University, 1094 Budapest, Hungary.; In Vivo Imaging Advanced Core Facility, Hungarian Center of Excellence for Molecular Medicine (HCEMM), 1094 Budapest, Hungary., Heydari F; Department of Biophysics and Radiation Biology, Semmelweis University, 1094 Budapest, Hungary., Szigeti K; Department of Biophysics and Radiation Biology, Semmelweis University, 1094 Budapest, Hungary., Máthé D; Department of Biophysics and Radiation Biology, Semmelweis University, 1094 Budapest, Hungary.; In Vivo Imaging Advanced Core Facility, Hungarian Center of Excellence for Molecular Medicine (HCEMM), 1094 Budapest, Hungary., Kristály F; Institute of Mineralogy and Geology, University of Miskolc, 3515 Miskolc, Hungary., Daróczi L; Department of Solid State Physics, University of Debrecen, P.O. Box 2, 4010 Debrecen, Hungary., Kaleta Z; Pro-Research Laboratory, Progressio Engineering Bureau Ltd., 8000 Szekesfehervar, Hungary.; Institute of Organic Chemistry, Semmelweis University, 1092 Budapest, Hungary., Viskolcz B; Institute of Chemistry, University of Miskolc, 3515 Miskolc, Hungary.; Higher Education and Industrial Cooperation Centre, University of Miskolc, 3515 Miskolc, Hungary., Nagy M; Institute of Chemistry, University of Miskolc, 3515 Miskolc, Hungary., Vanyorek L; Institute of Chemistry, University of Miskolc, 3515 Miskolc, Hungary. |
| Abstrakt: |
The need for stable and well-defined magnetic nanoparticles is constantly increasing in biomedical applications; however, their preparation remains challenging. We used two different solvothermal methods (12 h reflux and a 4 min microwave, MW) to synthesize amine-functionalized zinc ferrite (ZnFe 2 O 4 -NH 2 ) superparamagnetic nanoparticles. The morphological features of the two ferrite samples were the same, but the average particle size was slightly larger in the case of MW activation: 47 ± 14 nm (Refl.) vs. 63 ± 20 nm (MW). Phase identification measurements confirmed the exclusive presence of zinc ferrite with virtually the same magnetic properties. The Refl. samples had a zeta potential of -23.8 ± 4.4 mV, in contrast to the +7.6 ± 6.8 mV measured for the MW sample. To overcome stability problems in the colloidal phase, the ferrite nanoparticles were embedded in polyvinylpyrrolidone and could be easily redispersed in water. Two PVP-coated zinc ferrite samples were administered (1 mg/mL ZnFe 2 O 4 ) in X BalbC mice and were compared as contrast agents in magnetic resonance imaging (MRI). After determining the r1/r2 ratio, the samples were compared to other commercially available contrast agents. Consistent with other SPION nanoparticles, our sample exhibits a concentrated presence in the hepatic region of the animals, with comparable biodistribution and pharmacokinetics suspected. Moreover, a small dose of 1.3 mg/body weight kg was found to be sufficient for effective imaging. It should also be noted that no toxic side effects were observed, making ZnFe 2 O 4 -NH 2 advantageous for pharmaceutical formulations. |
