| Autor: |
Baidamshina DR; Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia., Trizna EY; Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia., Goncharova SS; Department of Biophysics and Biotechnology, Voronezh State University, 394018 Voronezh, Russia., Sorokin AV; Department of Biophysics and Biotechnology, Voronezh State University, 394018 Voronezh, Russia.; Laboratory of Bioresource Potential of Coastal Area, Institute for Advanced Studies, Sevastopol State University, 299053 Sevastopol, Russia., Lavlinskaya MS; Department of Biophysics and Biotechnology, Voronezh State University, 394018 Voronezh, Russia.; Laboratory of Bioresource Potential of Coastal Area, Institute for Advanced Studies, Sevastopol State University, 299053 Sevastopol, Russia., Melnik AP; Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia., Gafarova LF; Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia., Kharitonova MA; Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia., Ostolopovskaya OV; Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia., Artyukhov VG; Department of Biophysics and Biotechnology, Voronezh State University, 394018 Voronezh, Russia., Sokolova EA; Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia., Holyavka MG; Department of Biophysics and Biotechnology, Voronezh State University, 394018 Voronezh, Russia.; Laboratory of Bioresource Potential of Coastal Area, Institute for Advanced Studies, Sevastopol State University, 299053 Sevastopol, Russia., Bogachev MI; Biomedical Engineering Research Centre, St. Petersburg Electrotechnical University, 197022 St. Petersburg, Russia., Kayumov AR; Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia.; Interdepartment Research Laboratory, Kazan State Academy of Veterinary Medicine Named after N. E. Bauman, 420029 Kazan, Russia., Zelenikhin PV; Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia. |
| Abstrakt: |
In the last decade, Ficin, a proteolytic enzyme extracted from the latex sap of the wild fig tree, has been widely investigated as a promising tool for the treatment of microbial biofilms, wound healing, and oral care. Here we report the antibiofilm properties of the enzyme immobilized on soluble carboxymethyl chitosan (CMCh) and CMCh itself. Ficin was immobilized on CMCh with molecular weights of either 200, 350 or 600 kDa. Among them, the carrier with a molecular weight of 200 kDa bound the maximum amount of enzyme, binding up to 49% of the total protein compared to 19-32% of the total protein bound to other CMChs. Treatment with pure CMCh led to the destruction of biofilms formed by Streptococcus salivarius , Streptococcus gordonii , Streptococcus mutans , and Candida albicans , while no apparent effect on Staphylococcus aureus was observed. A soluble Ficin was less efficient in the destruction of the biofilms formed by Streptococcus sobrinus and S. gordonii . By contrast, treatment with CMCh200-immobilized Ficin led to a significant reduction of the biofilms of the primary colonizers S. gordonii and S. mutans. In model biofilms obtained by the inoculation of swabs from teeth of healthy volunteers, the destruction of the biofilm by both soluble and immobilized Ficin was observed, although the degree of the destruction varied between artificial plaque samples. Nevertheless, combined treatment of oral Streptococci biofilm by enzyme and chlorhexidine for 3 h led to a significant decrease in the viability of biofilm-embedded cells, compared to solely chlorhexidine application. This suggests that the use of either soluble or immobilized Ficin would allow decreasing the amount and/or concentration of the antiseptics required for oral care or improving the efficiency of oral cavity sanitization. |
