| Autor: |
Lovatto M; Departamento de Agronomia, Universidade Estadual de Maringá, Maringá 87020-900, Brazil., Gonçalves-Vidigal MC; Departamento de Agronomia, Universidade Estadual de Maringá, Maringá 87020-900, Brazil., Vaz Bisneta M; Departamento de Agronomia, Universidade Estadual de Maringá, Maringá 87020-900, Brazil., Calvi AC; Departamento de Agronomia, Universidade Estadual de Maringá, Maringá 87020-900, Brazil., Mazucheli J; Departamento de Estatística, Universidade Estadual de Maringá, Maringá 87020-900, Brazil., Vidigal Filho PS; Departamento de Agronomia, Universidade Estadual de Maringá, Maringá 87020-900, Brazil., Miranda EGR; Departamento de Agronomia, Universidade Estadual de Maringá, Maringá 87020-900, Brazil., Melotto M; Department of Plant Sciences, University of California, Davis, CA 95616, USA. |
| Abstrakt: |
Anthracnose (ANT) and angular leaf spot (ALS) are significant diseases in common bean, leading to considerable yield losses under specific environmental conditions. The California Dark Red Kidney (CDRK) bean cultivar is known for its resistance to multiple races of both pathogens. Previous studies have identified the CoPv01 CDRK /PhgPv01 CDRK resistance loci on chromosome Pv01. Here, we evaluated the expression levels of ten candidate genes near the CoPv01 CDRK /PhgPv01 CDRK loci and plant defense genes using quantitative real-time PCR in CDRK cultivar inoculated with races 73 of Colletotrichum lindemuthianum and 63-39 of Pseudocercospora griseola . Gene expression analysis revealed that the Phvul.001G246300 gene exhibited the most elevated levels, showing remarkable 7.8-fold and 8.5-fold increases for ANT and ALS, respectively. The Phvul.001G246300 gene encodes an abscisic acid (ABA) receptor with pyrabactin resistance, PYR1-like (PYL) protein, which plays a central role in the crosstalk between ABA and jasmonic acid responses. Interestingly, our results also showed that the other defense genes were initially activated. These findings provide critical insights into the molecular mechanisms underlying plant defense against these diseases and could contribute to the development of more effective disease management strategies in the future. |
