| Autor: |
Mizaeva T; G. N. Gabrichevsky Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia., Alieva K; G. N. Gabrichevsky Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia., Zulkarneev E; Plague Control Center, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, 119121 Moscow, Russia., Kurpe S; Institute of Biochemistry after H.Buniatyan National Academy of Sciences of the Republic of Armenia, Yerevan 0014, Armenia., Isakova K; Northern Water Problems Institute of the Karelian Research Centre of the Russian Academy of Sciences, 185000 Petrozavodsk, Republic of Karelia, Russia., Matrosova S; Institute of Biology, Ecology and Agricultural Technologies of the Petrozavodsk State University, 185000 Petrozavodsk, Republic of Karelia, Russia., Borvinskaya E; Institute of Biology, Irkutsk State University, 664003 Irkutsk, Russia., Sukhovskaya I; Institute of Biology, Ecology and Agricultural Technologies of the Petrozavodsk State University, 185000 Petrozavodsk, Republic of Karelia, Russia.; Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, 185000 Petrozavodsk, Republic of Karelia, Russia. |
| Abstrakt: |
The objective of this study was to investigate the bactericidal activity of blood plasma from cultured rainbow trout obtained from two different fish farms. Plasma from trout naturally infected with the bacterial pathogen Flavobacterium psychrophilum was found to inhibit the growth of Aeromonas hydrophila in vitro. Incubation of A. hydrophila in bacteriostatic trout plasma resulted in agglutination and growth retardation, without causing massive damage to the cell membrane. The proteome of the plasma with high antimicrobial activity revealed an abundance of high-density apolipoproteins, some isoforms of immunoglobulins, complement components C1q and C4, coagulation factors, lectins, periostin, and hemoglobin. Analysis of trout proteins retained on A. hydrophila cells revealed the presence of fish immunoglobulins, lectins, and complement components on bacteria whose growth was inhibited, although the native membrane attack complex of immunised trout plasma did not assemble effectively, resulting in a weak bactericidal effect. Furthermore, this study examined the bacterial response to trout plasma and suggested that the protein synthesis pathway was the target of antimicrobial proteins from fish blood. Taken together, these findings illustrate the advantages of the affinity approach for understanding the role of plasma proteins in host defence against pathogens. |
