| Autor: |
de Mello F; Department of Physiology, Bioscience Institute, University of Sao Paulo (IB/USP), 101 Matão Street, Travessa 14, Butantã District, São Paulo 05508-090, SP, Brazil., Alonso DJ; Department of Physiology, Bioscience Institute, University of Sao Paulo (IB/USP), 101 Matão Street, Travessa 14, Butantã District, São Paulo 05508-090, SP, Brazil., Faria NPVM; Department of Physiology, Bioscience Institute, University of Sao Paulo (IB/USP), 101 Matão Street, Travessa 14, Butantã District, São Paulo 05508-090, SP, Brazil., Marques VH; Department of Physiology, Bioscience Institute, University of Sao Paulo (IB/USP), 101 Matão Street, Travessa 14, Butantã District, São Paulo 05508-090, SP, Brazil., Oliveira EF; Aquaculture Center, São Paulo State University 'Julio de Mesquita Filho' (CAUNESP), Access Road Professor Paulo Donato Castellane, Vila Industrial District, Jaboticabal 14884-900, SP, Brazil., de Mello PH; Beacon Development, King Abdullah University of Science and Technology, 2713, Jeddah 23955, Saudi Arabia., Godoy LC; Department of Animal Science, Federal University of Rio Grande do Sul, 7712 Bento Gonçalves Avenue, Agronomia District, Porto Alegre 91540-000, RS, Brazil., Moreira RG; Department of Physiology, Bioscience Institute, University of Sao Paulo (IB/USP), 101 Matão Street, Travessa 14, Butantã District, São Paulo 05508-090, SP, Brazil. |
| Abstrakt: |
The vitrification of ovarian follicles is a strategic tool that may contribute to advances in aquaculture and the conservation of many important species. Despite the difficulties inherent to the cryopreservation of oocytes, some successful protocols have been developed for different species, but little is known about the capacity of oocytes to develop after thawing. Therefore, the profiles of the reproductive pathway genes and fatty acid membrane composition during the initial stages of development were analyzed in fresh ovarian follicles and follicles after the vitrification process. There were differences in the expression of the hypothalamic-pituitary-gonad axis genes during the follicular development in the control group as well as in the vitrified group. Similarly, alterations in the composition of fatty acids were observed after vitrification. Despite this, many alterations were observed in the vitrified group; more than half of the stage III ovarian follicles were able to grow and mature in vitro. Therefore, the vitrification of ovarian follicles may impact them at molecular and membrane levels, but it does not compromise their capability for in vitro maturation, which indicates that the technique can be a strategic tool for aquaculture. |
