From Pediatric to Adult Brain Cancer: Exploring Histone H3 Mutations in Australian Brain Cancer Patients.

Autor: Grebstad Tune B; Department of Pediatric Research, Division of Paediatric and Adolescent Medicine, Oslo University Hospital Rikshospitalet, 0372 Oslo, Norway.; Institute of Clinical Medicine, University of Oslo, 0318 Oslo, Norway.; Centre for Circulating Tumour Cell Diagnostics and Research, Ingham Institute for Applied Medical Research, 1 Campbell St, Liverpool, NSW 2170, Australia., Sareen H; Centre for Circulating Tumour Cell Diagnostics and Research, Ingham Institute for Applied Medical Research, 1 Campbell St, Liverpool, NSW 2170, Australia.; South Western Sydney Clinical School, University of New South Wales, Goulburn St, Liverpool, NSW 2170, Australia., Powter B; Centre for Circulating Tumour Cell Diagnostics and Research, Ingham Institute for Applied Medical Research, 1 Campbell St, Liverpool, NSW 2170, Australia., Kahana-Edwin S; Children's Cancer Research Unit, Kids Research, The Children's Hospital at Westmead, Westmead, NSW 2145, Australia., Cooper A; Centre for Circulating Tumour Cell Diagnostics and Research, Ingham Institute for Applied Medical Research, 1 Campbell St, Liverpool, NSW 2170, Australia.; School of Medicine, Western Sydney University, Campbelltown, NSW 2560, Australia., Koh ES; South Western Sydney Clinical School, University of New South Wales, Goulburn St, Liverpool, NSW 2170, Australia.; Department of Radiation Oncology, Liverpool Hospital, Liverpool, NSW 2170, Australia., Lee CS; School of Medicine, Western Sydney University, Campbelltown, NSW 2560, Australia.; Department of Radiation Oncology, Liverpool Hospital, Liverpool, NSW 2170, Australia., Po JW; Centre for Circulating Tumour Cell Diagnostics and Research, Ingham Institute for Applied Medical Research, 1 Campbell St, Liverpool, NSW 2170, Australia., McCowage G; Cancer Centre for Children, The Children Hospital at Westmead, Westmead, NSW 2145, Australia., Dexter M; Neurosurgery, The Children Hospital at Westmead, Westmead, NSW 2145, Australia., Cain L; Cancer Centre for Children, The Children Hospital at Westmead, Westmead, NSW 2145, Australia., O'Neill G; Children's Cancer Research Unit, Kids Research, The Children's Hospital at Westmead, Westmead, NSW 2145, Australia.; The University of Sydney Children's Hospital Westmead Clinical School, Faculty of Medicine & Health, The University of Sydney, Westmead, NSW 2145, Australia., Prior V; Children's Cancer Research Unit, Kids Research, The Children's Hospital at Westmead, Westmead, NSW 2145, Australia.; The University of Sydney Children's Hospital Westmead Clinical School, Faculty of Medicine & Health, The University of Sydney, Westmead, NSW 2145, Australia., Karpelowsky J; Children's Cancer Research Unit, Kids Research, The Children's Hospital at Westmead, Westmead, NSW 2145, Australia.; Paediatric Oncology and Thoracic Surgery, The Children's Hospital at Westmead, Westmead, NSW 2145, Australia.; Division of Child and Adolescent Health, The University of Sydney, Camperdown, NSW 2050, Australia., Tsoli M; Children's Cancer Institute, Randwick, NSW 2031, Australia., Baumbusch LO; Department of Pediatric Research, Division of Paediatric and Adolescent Medicine, Oslo University Hospital Rikshospitalet, 0372 Oslo, Norway.; Faculty of Health, Welfare and Organization, Østfold University College, 1757 Halden, Norway., Ziegler D; Children's Cancer Institute, Randwick, NSW 2031, Australia.; Kids Cancer Centre, Sydney Children's Hospital, Randwick, NSW 2052, Australia.; School of Clinical Medicine, UNSW Medicine & Health, UNSW Sydney, Kensington, NSW 2052, Australia., Roberts TL; Centre for Circulating Tumour Cell Diagnostics and Research, Ingham Institute for Applied Medical Research, 1 Campbell St, Liverpool, NSW 2170, Australia.; South Western Sydney Clinical School, University of New South Wales, Goulburn St, Liverpool, NSW 2170, Australia.; School of Medicine, Western Sydney University, Campbelltown, NSW 2560, Australia., DeSouza P; Centre for Circulating Tumour Cell Diagnostics and Research, Ingham Institute for Applied Medical Research, 1 Campbell St, Liverpool, NSW 2170, Australia.; School of Medicine, Western Sydney University, Campbelltown, NSW 2560, Australia., Becker TM; Centre for Circulating Tumour Cell Diagnostics and Research, Ingham Institute for Applied Medical Research, 1 Campbell St, Liverpool, NSW 2170, Australia.; South Western Sydney Clinical School, University of New South Wales, Goulburn St, Liverpool, NSW 2170, Australia.; School of Medicine, Western Sydney University, Campbelltown, NSW 2560, Australia., Ma Y; Centre for Circulating Tumour Cell Diagnostics and Research, Ingham Institute for Applied Medical Research, 1 Campbell St, Liverpool, NSW 2170, Australia.; South Western Sydney Clinical School, University of New South Wales, Goulburn St, Liverpool, NSW 2170, Australia.; School of Medicine, Western Sydney University, Campbelltown, NSW 2560, Australia.
Jazyk: angličtina
Zdroj: Biomedicines [Biomedicines] 2023 Oct 27; Vol. 11 (11). Date of Electronic Publication: 2023 Oct 27.
DOI: 10.3390/biomedicines11112907
Abstrakt: Genetic histone variants have been implicated in cancer development and progression. Mutations affecting the histone 3 (H3) family, H3.1 (encoded by HIST1H3B and HIST1H3C ) and H3.3 (encoded by H3F3A ), are mainly associated with pediatric brain cancers. While considered poor prognostic brain cancer biomarkers in children, more recent studies have reported H3 alterations in adult brain cancer as well. Here, we established reliable droplet digital PCR based assays to detect three histone mutations (H3.3-K27M, H3.3-G34R, and H3.1-K27M) primarily linked to childhood brain cancer. We demonstrate the utility of our assays for sensitively detecting these mutations in cell-free DNA released from cultured diffuse intrinsic pontine glioma (DIPG) cells and in the cerebral spinal fluid of a pediatric patient with DIPG. We further screened tumor tissue DNA from 89 adult patients with glioma and 1 with diffuse hemispheric glioma from Southwestern Sydney, Australia, an ethnically diverse region, for these three mutations. No histone mutations were detected in adult glioma tissue, while H3.3-G34R presence was confirmed in the diffuse hemispheric glioma patient.
Databáze: MEDLINE