The ameliorative effect of cerium oxide nanoparticles on chlorpyrifos induced hepatotoxicity in a rat model: Biochemical, molecular and immunohistochemical study.

Autor: Abdel-Karim RI; Forensic Medicine and Clinical Toxicology department, Faculty of Medicine, Suez Canal University, Egypt., Hashish RK; Forensic Medicine and Clinical Toxicology department, Faculty of Medicine, Suez Canal University, Egypt., Badran DI; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Egypt; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Badr University in Cairo, Cairo, Egypt. Electronic address: dalia_badran@med.suez.edu.eg., Mohammed SS; Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, Egypt., Salem NA; Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Egypt.
Jazyk: angličtina
Zdroj: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [J Trace Elem Med Biol] 2024 Jan; Vol. 81, pp. 127346. Date of Electronic Publication: 2023 Nov 20.
DOI: 10.1016/j.jtemb.2023.127346
Abstrakt: Background: Chlorpyrifos (CPF) is a widely used insecticide that causes toxicity to living organisms through the production of free radicals. Cerium oxide nanoparticles (CeO 2 NPs) are a new antioxidant agent that has proved therapeutic effects. We evaluated the effect of CeO 2 NPs on CPF hepatotoxicity.
Methods: Forty rats were randomized into four groups. Group I: rats received 1 ml corn oil by gastric tube once daily and 0.5 ml PBS by intra-peritoneal injection twice a week for 4 weeks. Group II: received CeO 2 NPs 0.5 mg/kg in PBS by i.p. injection, twice weekly for four weeks. Group III: were treated with oral administration of CPF 13.5 mg/kg in corn oil daily for 4 weeks. Group IV: received CPF as in group III, then each animal received CeO 2 NPs twice weekly for four weeks as in group II. Twenty-four hours after the last dose, rats were anesthetized and sera were collected for liver enzymes assessment. Afterwards, rats were sacrificed, livers were excised, the right lobe of each liver was fixed for immunohistochemical studies, and the left lobe was homogenized for oxidative profile assessment and molecular analysis.
Results: CPF group showed significant increase in liver transaminases, disturbance of the oxidative profile with up-regulation of BAX expression and down-regulation in the Bcl-2, Gadd45 and NFE2L2. CPF caused severe histopathological liver damage as well as significant increase in anti-Caspase 3 and TNF immunostaining. The CeO 2 NPs treated group revealed significant improvement of all previous parameters.
Conclusion: CeO 2 NPs could alleviate CPF hepatoxicity through decreasing expression of the inflammatory and apoptotic proteins and increasing the activity of antioxidant enzymes.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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Databáze: MEDLINE
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