| Autor: |
Ben Hassouna K; Laboratory of Genetic, Biodiversity and Bio-Resources Valorisation, High Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia.; Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy, University of Monastir, Monastir 5000, Tunisia., Hamed AM; Dairy Science Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt., Salah-Abbès JB; Laboratory of Genetic, Biodiversity and Bio-Resources Valorisation, High Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia., Chaieb K; Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy, University of Monastir, Monastir 5000, Tunisia., Abbès S; Laboratory of Genetic, Biodiversity and Bio-Resources Valorisation, High Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia., García-Campaña AM; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, 18071 Granada, Spain., Gámiz-Gracia L; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, 18071 Granada, Spain. |
| Abstrakt: |
Food and feed contamination with mycotoxins is a major public health concern. Humans and animals are exposed to these toxins by consuming contaminated products throughout their lives. In this study, a method based on dispersive liquid-liquid microextraction (DLLME), followed by liquid chromatography with fluorescence detection (LC-FLD), was validated for the determination of aflatoxins (AFs) M1, B1, B2, G1, G2, zearalenone (ZEN), and ochratoxin A (OTA). The method was applied to 150 raw cow milk samples and 90 market durum wheat samples from two Tunisian climatic regions: the littoral region (Mahdia) and the continental region (Béja). This work was carried out to obtain more surveillance data to support rapid initiatives to assure safe foods and protect consumer health and to estimate the daily exposure of the Tunisian population consuming those products. AFG2 and OTA were found in wheat with incidences of 54.4 and 11.1%, respectively. On the other side, milk samples were contaminated by AFG2, AFB1, and AFB2 with incidences of 8.7%, 2.0%, and 0.67%, respectively. Some of the samples showed OTA concentrations above the maximum limit allowed by the European Union, which represents a health risk for consumers in Tunisia, where no legislation exists about the maximum content of mycotoxins in food. |
