| Autor: |
Lee WK; Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), 2670 Iljudong-ro, Gujwa-eup, Jeju-si 63349, Republic of Korea.; Department of Marine Biotechnology, KIOST School, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea., Ryu YK; Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), 2670 Iljudong-ro, Gujwa-eup, Jeju-si 63349, Republic of Korea.; Department of Marine Biotechnology, KIOST School, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea., Kim T; Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), 2670 Iljudong-ro, Gujwa-eup, Jeju-si 63349, Republic of Korea., Park A; Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), 2670 Iljudong-ro, Gujwa-eup, Jeju-si 63349, Republic of Korea., Lee YJ; Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), 2670 Iljudong-ro, Gujwa-eup, Jeju-si 63349, Republic of Korea., Sunwoo IY; Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), 2670 Iljudong-ro, Gujwa-eup, Jeju-si 63349, Republic of Korea., Koh EJ; Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), 2670 Iljudong-ro, Gujwa-eup, Jeju-si 63349, Republic of Korea., Oh C; Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), 2670 Iljudong-ro, Gujwa-eup, Jeju-si 63349, Republic of Korea.; Department of Marine Biotechnology, KIOST School, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea., Choi WY; Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), 2670 Iljudong-ro, Gujwa-eup, Jeju-si 63349, Republic of Korea.; Department of Marine Biotechnology, KIOST School, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea., Kang DH; Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), 2670 Iljudong-ro, Gujwa-eup, Jeju-si 63349, Republic of Korea. |
| Abstrakt: |
Microalgae have gained attention as a promising source of chlorophylls and carotenoids in various industries. However, scaling up of conventional bubble columns presents challenges related to cell sedimentation and the presence of non-photosynthetic cells due to non-circulating zones and decreased light accessibility, respectively. Therefore, this study aimed to evaluate the newly developed continuously circulated bioreactor ROSEMAX at both laboratory and pilot scales, compared to a conventional bubble column. There was no significant difference in the biomass production and photosynthetic pigment content of Tetraselmis sp. cultivated at the laboratory scale ( p > 0.05). However, at the pilot scale, the biomass cultured in ROSEMAX showed significantly high biomass (1.69 ± 0.11 g/L, dry weight, DW), chlorophyll- a (14.60 ± 0.76 mg/g, DW), and total carotene (5.64 ± 0.81 mg/g, DW) concentrations compared to the conventional bubble column (1.17 ± 0.11 g/L, DW, 10.67 ± 0.72 mg/g, DW, 3.21 ± 0.56 mg/g, DW, respectively) ( p ≤ 0.05). Flow cytometric analyses confirmed that the proportion of Tetraselmis sp. live cells in the culture medium of ROSEMAX was 32.90% higher than that in the conventional bubble column, with a photosynthetic efficiency 1.14 times higher. These results support suggestions to use ROSEMAX as a bioreactor for industrial-scale applications. |
