| Autor: |
Goncalves BG; Department of Chemistry, Fordham University, 441 East Fordham Road, Bronx, New York, NY 10458, USA., Heise RM; Department of Chemistry, Fordham University, 441 East Fordham Road, Bronx, New York, NY 10458, USA., Banerjee IA; Department of Chemistry, Fordham University, 441 East Fordham Road, Bronx, New York, NY 10458, USA. |
| Jazyk: |
angličtina |
| Zdroj: |
Biomimetics (Basel, Switzerland) [Biomimetics (Basel)] 2023 Nov 14; Vol. 8 (7). Date of Electronic Publication: 2023 Nov 14. |
| DOI: |
10.3390/biomimetics8070548 |
| Abstrakt: |
Development of biocomposite scaffolds has gained tremendous attention due to their potential for tissue regeneration. However, most scaffolds often contain animal-derived collagen that may elicit an immunological response, necessitating the development of new biomaterials. Herein, we developed a new collagen-like peptide,(Pro-Ala-His) 10 (PAH) 10 , and explored its ability to be utilized as a functional biomaterial by incorporating it with a newly synthesized peptide-based self-assembled gel. The gel was prepared by conjugating a pectin derivative, galataric acid, with a pro-angiogenic peptide (LHYQDLLQLQY) and further functionalized with a cortistatin-derived peptide, (Phe-Trp-Lys-Thr) 4 (FWKT) 4 , and the bio-ionic liquid choline acetate. The self-assembly of (PAH) 10 and its interactions with the galactarate-peptide conjugates were examined using replica exchange molecular dynamics (REMD) simulations. Results revealed the formation of a multi-layered scaffold, with enhanced stability at higher temperatures. We then synthesized the scaffold and examined its physicochemical properties and its ability to integrate with aortic smooth muscle cells. The scaffold was further utilized as a bioink for bioprinting to form three-dimensional cell-scaffold matrices. Furthermore, the formation of actin filaments and elongated cell morphology was observed. These results indicate that the (PAH) 10 hybrid scaffold provides a suitable environment for cell adhesion, proliferation and growth, making it a potentially valuable biomaterial for tissue engineering. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
|
Nepřihlášeným uživatelům se plný text nezobrazuje |
K zobrazení výsledku je třeba se přihlásit.
|
