| Autor: |
Kaci K; HCTLab, Escuela Politécnica Superior, Universidad Autónoma de Madrid, 28049 Madrid, Spain., Enebral-Romero E; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, 28049 Madrid, Spain.; IMDEA-Nanociencia, Ciudad Universitaria de Cantoblanco, 28049 Madrid, Spain., Martínez-Periñán E; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, 28049 Madrid, Spain.; Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, 28049 Madrid, Spain., Garrido M; IMDEA-Nanociencia, Ciudad Universitaria de Cantoblanco, 28049 Madrid, Spain., Pérez EM; IMDEA-Nanociencia, Ciudad Universitaria de Cantoblanco, 28049 Madrid, Spain., López-Diego D; Instituto de Micro y Nanotecnología IMN-CNM, CSIC (CEI UAM+CSIC), 28760 Madrid, Spain., Luna M; Instituto de Micro y Nanotecnología IMN-CNM, CSIC (CEI UAM+CSIC), 28760 Madrid, Spain., González-de-Rivera G; HCTLab, Escuela Politécnica Superior, Universidad Autónoma de Madrid, 28049 Madrid, Spain., García-Mendiola T; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, 28049 Madrid, Spain.; Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, 28049 Madrid, Spain., Lorenzo E; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, 28049 Madrid, Spain.; IMDEA-Nanociencia, Ciudad Universitaria de Cantoblanco, 28049 Madrid, Spain.; Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, 28049 Madrid, Spain. |
| Abstrakt: |
An advanced, cost-effective, and portable DNA biosensor capable of detecting multiple bacteria simultaneously has been developed. The biosensor comprises a fast and inexpensive potentiostat that controls the applied potential to a screen-printed electrochemical array platform functionalized with MoS 2 flakes and bacterial DNA probes. The current response obtained by à la carte thionine functionalized carbon nanodots (Ty-CDs) is monitored as an electrochemical indicator of the hybridization event. The design of the potentiostat prioritizes achieving an optimal signal-to-noise ratio and incorporates a user-friendly interface compatible with various devices, including computers, mobile phones, and tablets. The device is compact, lightweight, and manufactured at a low cost. The key components of the potentiostat include a data acquisition board capable of analyzing multiple samples simultaneously and a controller board. The results of this study confirm the ability of the multiplex portable biosensor to successfully detect specific bacterial DNA sequences, demonstrating its reliability and superior performance compared with a traditional, more complex, and laboratory-oriented potentiostat. |
