Poly(Aspartic Acid) Promotes Odontoblast-like Cell Differentiation in Rat Molars with Exposed Pulp.
| Autor: | Dos Santos FFV; Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo 05508-000, SP, Brazil., Habelitz S; Department of Preventive and Restorative Dental Sciences, School of Dentistry, University of California, San Francisco, CA 94143, USA., Nascimento FD; Molecular Biology Division, Department of Biochemistry, Federal University of São Paulo, São Paulo 04044-020, SP, Brazil., Arana-Chavez VE; Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo 05508-000, SP, Brazil., Braga RR; Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo 05508-000, SP, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of functional biomaterials [J Funct Biomater] 2023 Nov 01; Vol. 14 (11). Date of Electronic Publication: 2023 Nov 01. |
| DOI: | 10.3390/jfb14110537 |
| Abstrakt: | In recent years, alternative pulpal therapies targeting dentinogenesis signaling pathways using different peptides have been investigated. The aim of this study was to verify the effectiveness of poly(aspartic acid), pAsp, in dentin regeneration using an animal model. Methods: Mechanical pulp exposure was performed in the upper molars of 56 Wistar rats, randomly divided as follows (n = 14): control (no treatment); MTA group-pulp capping with mineral trioxide aggregate (MTA Angelus); pAsp group-application of 20 μL of pAsp solution (25 mg·mL -1 ); MTA+pAsp group-application of MTA mixed with pAsp (5:1 by mass). Animals were euthanized after 7 or 21 days. Histological sections were submitted to hematoxylin-eosin and Brown and Brenn staining and immunohistochemical analysis for osteopontin (OPN) and dentin matrix protein 1 (DMP 1). Results: At 7 days, an acute inflammatory infiltrate and the presence of disorganized mineralized tissue were observed in all groups. At 21 days, the quality and thickness of the reparative dentin in treated groups were superior to the control, and bacterial contamination was observed in two MTA-pAsp specimens. While all treated groups showed intense immunostaining for OPN at 21 days, only the pAsp group expressed DMP 1, indicating the presence of fully differentiated odontoblast-like cells. Conclusion: Poly(aspartic) acid promoted dentin regeneration in rat molars in the absence of an additional calcium source and may be an alternative to MTA as a pulp-capping agent. |
| Databáze: | MEDLINE |
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