Dynamical control enables the formation of demixed biomolecular condensates.

Autor: Lin AZ; Division of Biology and Biomedical Sciences, Plant and Microbial Biosciences, Washington University in St. Louis, St. Louis, MO, 63130, USA., Ruff KM; Department of Biomedical Engineering and Center for Biomolecular Condensates, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, 63130, USA., Dar F; Department of Biomedical Engineering and Center for Biomolecular Condensates, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, 63130, USA., Jalihal A; Department of Cell Biology, Duke University, Durham, NC, 27708, USA., King MR; Department of Biomedical Engineering and Center for Biomolecular Condensates, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, 63130, USA., Lalmansingh JM; Department of Biomedical Engineering and Center for Biomolecular Condensates, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, 63130, USA., Posey AE; Department of Biomedical Engineering and Center for Biomolecular Condensates, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, 63130, USA., Erkamp NA; Department of Biomedical Engineering and Center for Biomolecular Condensates, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, 63130, USA.; Yusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK., Seim I; Department of Cell Biology, Duke University, Durham, NC, 27708, USA., Gladfelter AS; Department of Cell Biology, Duke University, Durham, NC, 27708, USA. amy.gladfelter@duke.edu., Pappu RV; Division of Biology and Biomedical Sciences, Plant and Microbial Biosciences, Washington University in St. Louis, St. Louis, MO, 63130, USA. pappu@wustl.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Nov 24; Vol. 14 (1), pp. 7678. Date of Electronic Publication: 2023 Nov 24.
DOI: 10.1038/s41467-023-43489-4
Abstrakt: Cellular matter can be organized into compositionally distinct biomolecular condensates. For example, in Ashbya gossypii, the RNA-binding protein Whi3 forms distinct condensates with different RNA molecules. Using criteria derived from a physical framework for explaining how compositionally distinct condensates can form spontaneously via thermodynamic considerations, we find that condensates in vitro form mainly via heterotypic interactions in binary mixtures of Whi3 and RNA. However, within these condensates, RNA molecules become dynamically arrested. As a result, in ternary systems, simultaneous additions of Whi3 and pairs of distinct RNA molecules lead to well-mixed condensates, whereas delayed addition of an RNA component results in compositional distinctness. Therefore, compositional identities of condensates can be achieved via dynamical control, being driven, at least partially, by the dynamical arrest of RNA molecules. Finally, we show that synchronizing the production of different RNAs leads to more well-mixed, as opposed to compositionally distinct condensates in vivo.
(© 2023. The Author(s).)
Databáze: MEDLINE
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