Evaluating the use of lake sedimentary DNA in palaeolimnology: A comparison with long-term microscopy-based monitoring of the phytoplankton community.

Autor: Thorpe AC; UK Centre for Ecology & Hydrology (UKCEH), Wallingford, UK.; School of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham, UK., Mackay EB; UK Centre for Ecology & Hydrology (UKCEH), Lancaster, UK., Goodall T; UK Centre for Ecology & Hydrology (UKCEH), Wallingford, UK., Bendle JA; School of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham, UK., Thackeray SJ; UK Centre for Ecology & Hydrology (UKCEH), Lancaster, UK., Maberly SC; UK Centre for Ecology & Hydrology (UKCEH), Lancaster, UK., Read DS; UK Centre for Ecology & Hydrology (UKCEH), Wallingford, UK.
Jazyk: angličtina
Zdroj: Molecular ecology resources [Mol Ecol Resour] 2024 Feb; Vol. 24 (2), pp. e13903. Date of Electronic Publication: 2023 Nov 23.
DOI: 10.1111/1755-0998.13903
Abstrakt: Palaeolimnological records provide valuable information about how phytoplankton respond to long-term drivers of environmental change. Traditional palaeolimnological tools such as microfossils and pigments are restricted to taxa that leave sub-fossil remains, and a method that can be applied to the wider community is required. Sedimentary DNA (sedDNA), extracted from lake sediment cores, shows promise in palaeolimnology, but validation against data from long-term monitoring of lake water is necessary to enable its development as a reliable record of past phytoplankton communities. To address this need, 18S rRNA gene amplicon sequencing was carried out on lake sediments from a core collected from Esthwaite Water (English Lake District) spanning ~105 years. This sedDNA record was compared with concurrent long-term microscopy-based monitoring of phytoplankton in the surface water. Broadly comparable trends were observed between the datasets, with respect to the diversity and relative abundance and occurrence of chlorophytes, dinoflagellates, ochrophytes and bacillariophytes. Up to 20% of genera were successfully captured using both methods, and sedDNA revealed a previously undetected community of phytoplankton. These results suggest that sedDNA can be used as an effective record of past phytoplankton communities, at least over timescales of <100 years. However, a substantial proportion of genera identified by microscopy were not detected using sedDNA, highlighting the current limitations of the technique that require further development such as reference database coverage. The taphonomic processes which may affect its reliability, such as the extent and rate of deposition and DNA degradation, also require further research.
(© 2023 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: