Milk fat miRNome changes in response to LPS challenge in Holstein cows.

Autor: Leroux C; INRAE, Université Clermont Auvergne, VetAgro Sup, UMR Herbivores, 63122, Saint-Genès-Champanelle, France. christine.leroux@inrae.fr., Cuccato M; INRAE, Université Clermont Auvergne, VetAgro Sup, UMR Herbivores, 63122, Saint-Genès-Champanelle, France.; Dipartimento di Scienze Veterinarie, Università degli Studi di Torino, Largo Paolo Braccini 2, 10095, Torino, Italy., Pawłowski K; INRAE, Université Clermont Auvergne, VetAgro Sup, UMR Herbivores, 63122, Saint-Genès-Champanelle, France.; Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw Univeristy of Life Sciences, Nowoursynowska 159c, 02-776, Warsaw, Poland., Cannizzo FT; Dipartimento di Scienze Veterinarie, Università degli Studi di Torino, Largo Paolo Braccini 2, 10095, Torino, Italy., Sacchi P; Dipartimento di Scienze Veterinarie, Università degli Studi di Torino, Largo Paolo Braccini 2, 10095, Torino, Italy., Pires JAA; INRAE, Université Clermont Auvergne, VetAgro Sup, UMR Herbivores, 63122, Saint-Genès-Champanelle, France., Faulconnier Y; INRAE, Université Clermont Auvergne, VetAgro Sup, UMR Herbivores, 63122, Saint-Genès-Champanelle, France.
Jazyk: angličtina
Zdroj: Veterinary research [Vet Res] 2023 Nov 22; Vol. 54 (1), pp. 111. Date of Electronic Publication: 2023 Nov 22.
DOI: 10.1186/s13567-023-01231-4
Abstrakt: Mastitis is an inflammatory disease in dairy cows, causing economic losses and reducing animal welfare. In order to contribute for the discovery of early and noninvasive indicators, our objective was to determine the effects of a lipopolysaccharide (LPS) challenge on the microRNA profile (miRNome) of milk fat, using microarray analyses in cows. Cows were fed a lactation diet at ad libitum intake (n = 6). At 27 ± 3 days in milk, cows were injected with 50 µg of LPS Escherichia coli in one healthy rear mammary quarter. Milk samples were collected just before LPS challenge (LPS-) and 6.5 h after LPS challenge (LPS +) from the same cows. Microarray analysis was performed using customized 8 × 60 K ruminant miRNA microarrays to compare LPS- to LPS + miRNome. In silico functional analyses were performed using OmicsNet and Mienturnet software. MiRNome comparison between LPS- and LPS + identified 37 differentially abundant miRNAs (q-value ≤ 0.05). The predicted target genes of the 37 differentially abundant miRNAs are mostly involved in cell life including apoptosis, cell cycle, proliferation and differentiation and in gene expression processes. MiRNome analyses suggest that miRNAs profile is related to the inflammation response of the mammary gland. In conclusion, we demonstrated that milk fat might be an easy and rapid source of miRNAs that are potential indicators of early mastitis in cows.
(© 2023. The Author(s).)
Databáze: MEDLINE
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