The influence of growth medium on the photodynamic susceptibility of Aggregatibacter actinomycetemcomitans to antimicrobial blue light.

Autor: Salviatto LTC; Biophotonics Applied to Health Science Postgraduate program, Nove de Julho UniversityUNINOVE, São Paulo, Brazil. lutticosta@gmail.com., Prates RA; Biophotonics Applied to Health Science Postgraduate program, Nove de Julho UniversityUNINOVE, São Paulo, Brazil., Pavani C; Biophotonics Applied to Health Science Postgraduate program, Nove de Julho UniversityUNINOVE, São Paulo, Brazil., Bussadori SK; Biophotonics Applied to Health Science Postgraduate program, Nove de Julho UniversityUNINOVE, São Paulo, Brazil., Deana AM; Biophotonics Applied to Health Science Postgraduate program, Nove de Julho UniversityUNINOVE, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Lasers in medical science [Lasers Med Sci] 2023 Nov 22; Vol. 38 (1), pp. 274. Date of Electronic Publication: 2023 Nov 22.
DOI: 10.1007/s10103-023-03937-5
Abstrakt: The aim of this study was to investigate whether antimicrobial blue light (aBL) can cause the death of Aggregatibacter actinomycetemcomitans (A.a) and to determine the influence of different culture media, specifically brain heart infusion and blood agar, on bacterial survival fraction. An LED emitting at 403 ± 15 nm, with a radiant power of 1W, irradiance of 588.2 mW/cm 2 , and an irradiation time of 0 min, 1 min, 5 min, 10 min, 30 min, and 60 min, was used. The plates were incubated in microaerophilic conditions at 37 °C for 48 h, and the colony-forming units were counted. The photosensitizers were investigated using spectroscopy and fluorescence microscopy. There was no significant difference between the culture media (p > 0.05). However, a statistical reduction in both media was observed at 30 min (1058 J/cm 2 ) (p < 0.05). The findings of this study suggest that aBL has the potential to kill bacteria regardless of the culture media used. Light therapy could be a promising and cost-effective strategy for preventing periodontal disease when used in combination with mechanical plaque control.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.)
Databáze: MEDLINE
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