Renal amyloid-A amyloidosis in cats: Characterization of proteinuria and biomarker discovery, and associations with kidney histology.

Autor: Palizzotto C; AniCura Istituto Veterinario Novara, Granozzo con Monticello, NO, Italy., Ferri F; AniCura Istituto Veterinario Novara, Granozzo con Monticello, NO, Italy.; Department of Animal Medicine, Production and Health, University of Padova, Legnaro, PD, Italy.; Studio Veterinario Associato Vet2Vet di Ferri e Porporato, Orbassano, TO, Italy., Callegari C; AniCura Istituto Veterinario Novara, Granozzo con Monticello, NO, Italy., Rossi F; AniCura Istituto Veterinario Novara, Granozzo con Monticello, NO, Italy., Manfredi M; Department of Translational Medicine, University of Piemonte Orientale, Novara, Italy., Carcangiu L; Department of Translational Medicine, University of Piemonte Orientale, Novara, Italy., Gerardi G; Department of Animal Medicine, Production and Health, University of Padova, Legnaro, PD, Italy., Ferro S; Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, PD, Italy., Cavicchioli L; Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, PD, Italy., Müller E; Laboklin, Laboratory for Clinical Diagnostics, Bad Kissingen, Germany., Weiss M; Laboklin, Laboratory for Clinical Diagnostics, Bad Kissingen, Germany., Vogt AC; Department of Rheumatology and Immunology, University Hospital Bern, Bern, Switzerland.; Department of BioMedical Research, University of Bern, Bern, Switzerland.; Graduate School for Cellular and Biomedical Sciences (GCB), University of Bern, Bern, Switzerland., Lavatelli F; Department of Molecular Medicine, University of Pavia, Pavia, Italy., Ricagno S; Institute of Molecular and Translational Cardiology, IRCCS Policlinico San Donato, Milan, Italy.; Department of Biosciences, Università degli Studi di Milano, Milan, Italy., Hurley K; Mars Veterinary Health, McLean, Virginia, USA., Zini E; AniCura Istituto Veterinario Novara, Granozzo con Monticello, NO, Italy.; Department of Animal Medicine, Production and Health, University of Padova, Legnaro, PD, Italy.; Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Jazyk: angličtina
Zdroj: Journal of veterinary internal medicine [J Vet Intern Med] 2024 Jan-Feb; Vol. 38 (1), pp. 205-215. Date of Electronic Publication: 2023 Nov 22.
DOI: 10.1111/jvim.16920
Abstrakt: Background: Amyloid A (AA) amyloidosis is a protein misfolding disease arising from serum amyloid A (SAA). Systemic AA amyloidosis recently was shown to have a high prevalence in shelter cats in Italy and was associated with azotemia and proteinuria.
Objectives: Investigate urine protein profiles and diagnostic biomarkers in cats with renal AA amyloidosis.
Animals: Twenty-nine shelter cats.
Methods: Case-control study. Cats with renal proteinuria that died or were euthanized between 2018 and 2021 with available necropsy kidney, liver and spleen samples, and with surplus urine collected within 30 days before death, were included. Histology was used to characterize renal damage and amyloid amount and distribution; immunohistochemistry was used to confirm AA amyloidosis. Urine protein-to-creatinine (UPC) and urine amyloid A-to-creatinine (UAAC) ratios were calculated, and sodium dodecyl sulfate-agarose gel electrophoresis (SDS-AGE) and liquid chromatography-mass spectrometry (LC-MS) of proteins were performed.
Results: Twenty-nine cats were included. Nineteen had AA amyloidosis with renal involvement. Cats with AA amyloidosis had a higher UPC (median, 3.9; range, 0.6-12.7 vs 1.5; 0.6-3.1; P = .03) and UAAC ratios (median, 7.18 × 10 -3 ; range, 23 × 10 -3 -21.29 × 10 -3 vs 1.26 × 10 -3 ; 0.21 × 10 -3 -6.33 × 10 -3 ; P = .04) than unaffected cats. The SDS-AGE identified mixed-type proteinuria in 89.4% of cats with AA amyloidosis and in 55.6% without AA amyloidosis (P = .57). The LC-MS identified 63 potential biomarkers associated with AA amyloidosis (P < .05). Among these, urine apolipoprotein C-III was higher in cats with AA amyloidosis (median, 1.38 × 10 7 ; range, 1.85 × 10 5 -5.29 × 10 7 vs 1.76 × 10 6 ; 0.0 × 10 0 -1.38 × 10 7 ; P = .01). In the kidney, AA-amyloidosis was associated with glomerulosclerosis (P = .02) and interstitial fibrosis (P = .05).
Conclusions and Clinical Importance: Renal AA amyloidosis is associated with kidney lesions, increased proteinuria and increased urine excretion of SAA in shelter cats. Additional studies are needed to characterize the role of lipid transport proteins in the urine of affected cats.
(© 2023 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.)
Databáze: MEDLINE
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