Anti-ribosomal P (anti-P) antibodies in patients with autoimmune hepatitis.

Autor: Gallo CA; Division of Gastroenterology , Universidade Federal de São Paulo , São Paulo , SP , Brazil ., Dellavance A; Research and Development Division , Grupo Fleury , São Paulo , SP , Brazil ., Gama RA; Division of Gastroenterology , Universidade Federal de São Paulo , São Paulo , SP , Brazil ., Silva AE; Division of Gastroenterology , Universidade Federal de São Paulo , São Paulo , SP , Brazil ., Silva ISSE; Division of Gastroenterology , Universidade Federal de São Paulo , São Paulo , SP , Brazil ., Andrade LEC; Division of Rheumatology , Universidade Federal de São Paulo , São Paulo , SP , Brazil .; Division of Immunology , Grupo Fleury , São Paulo , SP , Brazil ., Ferraz MLG; Division of Gastroenterology , Universidade Federal de São Paulo , São Paulo , SP , Brazil .
Jazyk: angličtina
Zdroj: Einstein (Sao Paulo, Brazil) [Einstein (Sao Paulo)] 2023 Nov 17; Vol. 21, pp. eAO0375. Date of Electronic Publication: 2023 Nov 17 (Print Publication: 2023).
DOI: 10.31744/einstein_journal/2023AO0375
Abstrakt: Objective: Anti-P-ribosomal antibody is a biomarker of systemic lupus erythematosus mainly associated with renal, nervous, and hepatic involvement. Systemic lupus erythematosus may present with features similar to autoimmune hepatitis. This study aimed to investigate the association of Anti-P-ribosomal antibodies in systemic lupus erythematosus compared to autoimmune hepatitis in the general Brazilian population. Autoimmune hepatitis and systemic lupus erythematosus share several clinical features. ۪Anti-P ribosomal antibody is a biomarker for systemic lupus erythematosus. The association between anti-P ribosomal antibody and autoimmune hepatitis has shown conflicting results. Our results showed no association between anti-P ribosomal antibody and autoimmune hepatitis. Published studies have shown associations between anti-ribosomal P (anti-P) antibody and systemic lupus erythematosus with hepatic manifestations. This has been reported also in autoimmune hepatitis. However, the consistency of the latter association remains controversial. This study aimed to evaluate the frequency of anti-P antibodies in autoimmune hepatitis using two different immunoassays.
Methods: One-hundred and seventy-seven patients with autoimmune hepatitis were screened, and 142 were analyzed for anti-P antibody positivity. The samples were first analyzed using two different immunoassays: enzyme-linked immunosorbent assay (ELISA) and chemiluminescence and then compared with a group of 60 patients with systemic lupus erythematous. The positive samples were subjected to western blot analysis.
Results: Anti-P was found in 5/142 autoimmune hepatitis cases (3.5%) by chemiluminescence and in none by ELISA. Among the five chemiluminescence-positive autoimmune hepatitis samples, on anti-P western blot analysis one was negative, two were weakly positive, and two were positive. In contrast, anti-P was detected in 10/60 patients with systemic lupus erythematosus (16.7%) and presented higher chemiluminescence units than the autoimmune hepatitis samples.
Conclusion: A low frequency of anti-P antibodies was observed in autoimmune hepatitis, suggesting that this test is not useful for the diagnosis or management of this disease.
Databáze: MEDLINE
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