Application of the Fpg-modified comet assay on three-spined stickleback in freshwater biomonitoring: toward a multi-biomarker approach of genotoxicity.

Autor: Cant A; Institut National de L'Environnement Industriel Et Des Risques (INERIS), UMR-I 02 SEBIO, Parc Technologique Alata, BP 2, 60550, Verneuil-en-Halatte, France.; Université de Reims Champagne-Ardenne (URCA), UMR-I 02 SEBIO, Moulin de La Housse, B.P. 1039, 51687, Reims, France., Bado-Nilles A; Institut National de L'Environnement Industriel Et Des Risques (INERIS), UMR-I 02 SEBIO, Parc Technologique Alata, BP 2, 60550, Verneuil-en-Halatte, France., Porcher JM; Institut National de L'Environnement Industriel Et Des Risques (INERIS), UMR-I 02 SEBIO, Parc Technologique Alata, BP 2, 60550, Verneuil-en-Halatte, France., Bolzan D; Agence de L'Eau Artois-Picardie, Centre Tertiaire de L'Arsenal, BP 80818, 59508, Douai Cedex, France., Prygiel J; Agence de L'Eau Artois-Picardie, Centre Tertiaire de L'Arsenal, BP 80818, 59508, Douai Cedex, France., Catteau A; Université de Reims Champagne-Ardenne (URCA), UMR-I 02 SEBIO, Moulin de La Housse, B.P. 1039, 51687, Reims, France., Turiès C; Institut National de L'Environnement Industriel Et Des Risques (INERIS), UMR-I 02 SEBIO, Parc Technologique Alata, BP 2, 60550, Verneuil-en-Halatte, France., Geffard A; Université de Reims Champagne-Ardenne (URCA), UMR-I 02 SEBIO, Moulin de La Housse, B.P. 1039, 51687, Reims, France., Bonnard M; Université de Reims Champagne-Ardenne (URCA), UMR-I 02 SEBIO, Moulin de La Housse, B.P. 1039, 51687, Reims, France. marc.bonnard@univ-reims.fr.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2023 Nov 22. Date of Electronic Publication: 2023 Nov 22.
DOI: 10.1007/s11356-023-30756-6
Abstrakt: Aquatic species are exposed to a wide spectrum of substances, which can compromise their genomic integrity by inducing DNA damage or oxidative stress. Genotoxicity biomarkers as DNA strand breaks and chromosomal damages developed on sentinel species have already proved to be relevant in aquatic biomonitoring. However, these biomarkers do not reflect DNA oxidative lesions, i.e., the 8-oxodG, recognized as pre-mutagenic lesion if not or mis-repaired in human biomonitoring. The relevance to include the measure of these lesions by using the Fpg-modified comet assay on erythrocytes of the three-spined stickleback was investigated. An optimization step of the Fpg-modified comet assay considering enzyme buffer impact, Fpg concentration, and incubation time has been performed. Then, this measure was integrated in a battery of genotoxicity and cytotoxicity biomarkers (considering DNA strand breaks, DNA content variation, and cell apoptosis/necrosis and density) and applied in a freshwater monitoring program on six stations of the Artois Picardie watershed (3-week caging of control fish). These biomarkers allowed to discriminate the stations regarding the genotoxic potential of water bodies and specifically by the measure of oxidative DNA lesions, which seem to be a promising tool in environmental genotoxicity risk assessment.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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