Condensin positioning at telomeres by shelterin proteins drives sister-telomere disjunction in anaphase.

Autor:	Colin L; CNRS - Laboratory of Biology and Modelling of the Cell, Lyon, France.; ENS de Lyon, Université Lyon, site Jacques Monod, Lyon, France., Reyes C; MCD, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, Toulouse, France., Berthezene J; MCD, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, Toulouse, France., Maestroni L; CNRS, INSERM, Aix Marseille Université, Institut Paoli-Calmettes, CRCM, Equipe labellisée par la Ligue Nationale contre le Cancer, Marseille, France., Modolo L; CNRS - Laboratory of Biology and Modelling of the Cell, Lyon, France.; ENS de Lyon, Université Lyon, site Jacques Monod, Lyon, France., Toselli E; CNRS - Laboratory of Biology and Modelling of the Cell, Lyon, France.; ENS de Lyon, Université Lyon, site Jacques Monod, Lyon, France., Chanard N; MCD, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, Toulouse, France.; CBI, MCD-UMR5077, CNRS, Chromatin Dynamics Team, Toulouse, France., Schaak S; MCD, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, Toulouse, France.; CBI, MCD-UMR5077, CNRS, Chromatin Dynamics Team, Toulouse, France., Cuvier O; MCD, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, Toulouse, France.; CBI, MCD-UMR5077, CNRS, Chromatin Dynamics Team, Toulouse, France., Gachet Y; MCD, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, Toulouse, France., Coulon S; CNRS, INSERM, Aix Marseille Université, Institut Paoli-Calmettes, CRCM, Equipe labellisée par la Ligue Nationale contre le Cancer, Marseille, France., Bernard P; CNRS - Laboratory of Biology and Modelling of the Cell, Lyon, France.; ENS de Lyon, Université Lyon, site Jacques Monod, Lyon, France., Tournier S; MCD, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, Toulouse, France.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2023 Nov 21; Vol. 12. Date of Electronic Publication: 2023 Nov 21.
DOI:	10.7554/eLife.89812
Abstrakt:	The localization of condensin along chromosomes is crucial for their accurate segregation in anaphase. Condensin is enriched at telomeres but how and for what purpose had remained elusive. Here, we show that fission yeast condensin accumulates at telomere repeats through the balancing acts of Taz1, a core component of the shelterin complex that ensures telomeric functions, and Mit1, a nucleosome remodeler associated with shelterin. We further show that condensin takes part in sister-telomere separation in anaphase, and that this event can be uncoupled from the prior separation of chromosome arms, implying a telomere-specific separation mechanism. Consistent with a cis-acting process, increasing or decreasing condensin occupancy specifically at telomeres modifies accordingly the efficiency of their separation in anaphase. Genetic evidence suggests that condensin promotes sister-telomere separation by counteracting cohesin. Thus, our results reveal a shelterin-based mechanism that enriches condensin at telomeres to drive in cis their separation during mitosis. Competing Interests: LC, CR, JB, LM, LM, ET, NC, SS, OC, YG, SC, PB, ST No competing interests declared (© 2023, Colin, Reyes et al.)
Databáze:	MEDLINE
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