Cellular Architects at Work: Cells Building their Own Microgel Houses.

Autor: Bulut S; DWI - Leibniz Institute for Interactive Materials e. V, RWTH Aachen University, Forckenbeckstraße 50, 52074, Aachen, Germany.; Functional and Interactive Polymers, Institute of Technical and Macromolecular Chemistry (ITMC), RWTH Aachen University, Worringerweg 2, 52074, Aachen, Germany., Günther D; DWI - Leibniz Institute for Interactive Materials e. V, RWTH Aachen University, Forckenbeckstraße 50, 52074, Aachen, Germany.; Advanced Materials for Biomedicine (AMB), Institute of Technical and Macromolecular Chemistry (ITMC), RWTH Aachen University, Worringerweg 2, 52074, Aachen, Germany.; Advanced Materials for Biomedicine (AMB), Institute of Applied Medical Engineering (AME), University Hospital RWTH Aachen, Center for Biohybrid Medical Systems (CBMS), Forckenbeckstraße 55, 52074, Aachen, Germany., Bund M; DWI - Leibniz Institute for Interactive Materials e. V, RWTH Aachen University, Forckenbeckstraße 50, 52074, Aachen, Germany.; Functional and Interactive Polymers, Institute of Technical and Macromolecular Chemistry (ITMC), RWTH Aachen University, Worringerweg 2, 52074, Aachen, Germany., Haats C; DWI - Leibniz Institute for Interactive Materials e. V, RWTH Aachen University, Forckenbeckstraße 50, 52074, Aachen, Germany.; Advanced Materials for Biomedicine (AMB), Institute of Technical and Macromolecular Chemistry (ITMC), RWTH Aachen University, Worringerweg 2, 52074, Aachen, Germany., Bissing T; DWI - Leibniz Institute for Interactive Materials e. V, RWTH Aachen University, Forckenbeckstraße 50, 52074, Aachen, Germany.; Functional and Interactive Polymers, Institute of Technical and Macromolecular Chemistry (ITMC), RWTH Aachen University, Worringerweg 2, 52074, Aachen, Germany., Bastard C; DWI - Leibniz Institute for Interactive Materials e. V, RWTH Aachen University, Forckenbeckstraße 50, 52074, Aachen, Germany.; Advanced Materials for Biomedicine (AMB), Institute of Technical and Macromolecular Chemistry (ITMC), RWTH Aachen University, Worringerweg 2, 52074, Aachen, Germany.; Advanced Materials for Biomedicine (AMB), Institute of Applied Medical Engineering (AME), University Hospital RWTH Aachen, Center for Biohybrid Medical Systems (CBMS), Forckenbeckstraße 55, 52074, Aachen, Germany., Wessling M; DWI - Leibniz Institute for Interactive Materials e. V, RWTH Aachen University, Forckenbeckstraße 50, 52074, Aachen, Germany.; Department of Chemical Process Engineering (AVT.CVT), RWTH Aachen University, Forckenbeckstraße 51, 52074, Aachen, Germany., De Laporte L; DWI - Leibniz Institute for Interactive Materials e. V, RWTH Aachen University, Forckenbeckstraße 50, 52074, Aachen, Germany.; Advanced Materials for Biomedicine (AMB), Institute of Technical and Macromolecular Chemistry (ITMC), RWTH Aachen University, Worringerweg 2, 52074, Aachen, Germany.; Advanced Materials for Biomedicine (AMB), Institute of Applied Medical Engineering (AME), University Hospital RWTH Aachen, Center for Biohybrid Medical Systems (CBMS), Forckenbeckstraße 55, 52074, Aachen, Germany., Pich A; DWI - Leibniz Institute for Interactive Materials e. V, RWTH Aachen University, Forckenbeckstraße 50, 52074, Aachen, Germany.; Functional and Interactive Polymers, Institute of Technical and Macromolecular Chemistry (ITMC), RWTH Aachen University, Worringerweg 2, 52074, Aachen, Germany.; Aachen Maastricht Institute for Biobased Materials (AMIBM), Maastricht University, Brightlands Chemelot Campus, Urmonderbaan 22, Geleen, 6167 RD, Netherlands.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2024 Oct; Vol. 13 (25), pp. e2302957. Date of Electronic Publication: 2023 Nov 28.
DOI: 10.1002/adhm.202302957
Abstrakt: Microporous annealed particle (MAP) scaffolds are investigated for their application as injectable 3D constructs in the field of regenerative medicine and tissue repair. While available MAP scaffolds provide a stable interlinked matrix of microgels for cell culture, the infiltration depth and space for cells to grow inside the scaffolds is pre-determined by the void fraction during the assembly. In the case of MAP scaffolds fabricated from interlinked spherical microgels, a cellularity gradient can be observed with the highest cell density on the scaffold surface. Additionally, the interlinked microgel network limits the ability of cells to remodel their environment, which contradicts native tissue dynamics. In this work, a cell-induced interlinking method for MAP scaffold formation is established, which avoids the necessity of chemical crosslinkers and pre-engineered pores to achieve micro- or macropores in these 3D frameworks. This method enables cells to self-organize with microgels into dynamic tissue constructs, which can be further controlled by altering the microgel properties, the cell/microgel ratio, and well shape. To form a cell-induced interlinked scaffold, the cells are mixed with dextran-based microgels and function as a glue between the microgels, resulting in a more homogenous cell distribution throughout the scaffold with efficient cell-cell interactions.
(© 2023 The Authors. Advanced Healthcare Materials published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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