Tick extracellular vesicles undermine epidermal wound healing during hematophagy.

Autor: Marnin L; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA., Valencia LM; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA., Bogale HN; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA., Laukaitis-Yousey HJ; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA., Rolandelli A; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA., Ferraz CR; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA., O'Neal AJ; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA., Schmitter-Sánchez AD; Division of Dermatology, Department of Medicine, College of Human Medicine, Michigan State University, East Lansing, MI, USA.; Department of Pharmacology and Toxicology, College of Human Medicine, Michigan State University, East Lansing, MI, USA.; Institute for Quantitative Health Science & Engineering, Michigan State University, East Lansing, MI, USA., Cuevas EB; Department of Veterinary Pathobiology, School of Veterinary Medicine and BiomedicalSciences, Texas A&M University, College Station, TX, USA., Nguyen TT; Department of Veterinary Pathobiology, School of Veterinary Medicine and BiomedicalSciences, Texas A&M University, College Station, TX, USA., Leal-Galvan B; Department of Entomology, Texas A&M University, College Station, TX, USA., Rickert DM; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA., Mendes MT; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA., Samaddar S; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA., Butler LR; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA., Singh N; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA., Cabrera Paz FE; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA., Oliver JD; Division of Environmental Health Sciences, School of Public Health, University of Minnesota, Minneapolis, MN, USA., Jameson JM; Department of Biology, California State University San Marcos, San Marcos, CA, USA., Munderloh UG; Department of Entomology, University of Minnesota, Minneapolis, MN, USA., Oliva Chávez AS; Department of Entomology, Texas A&M University, College Station, TX, USA., Mulenga A; Department of Veterinary Pathobiology, School of Veterinary Medicine and BiomedicalSciences, Texas A&M University, College Station, TX, USA., Park S; Division of Dermatology, Department of Medicine, College of Human Medicine, Michigan State University, East Lansing, MI, USA.; Department of Pharmacology and Toxicology, College of Human Medicine, Michigan State University, East Lansing, MI, USA.; Institute for Quantitative Health Science & Engineering, Michigan State University, East Lansing, MI, USA., Serre D; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA.; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA., Pedra JHF; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA.
Jazyk: angličtina
Zdroj: BioRxiv : the preprint server for biology [bioRxiv] 2024 Sep 18. Date of Electronic Publication: 2024 Sep 18.
DOI: 10.1101/2023.11.10.566612
Abstrakt: Wound healing has been extensively studied through the lens of inflammatory disorders and cancer, but limited attention has been given to hematophagy and arthropod-borne diseases. Hematophagous ectoparasites, including ticks, subvert the wound healing response to maintain prolonged attachment and facilitate blood-feeding. Here, we unveil a strategy by which extracellular vesicles (EVs) ensure blood-feeding and arthropod survival in three medically relevant tick species. We demonstrate through single cell RNA sequencing and murine genetics that wildtype animals infested with EV-deficient Ixodes scapularis display a unique population of keratinocytes with an overrepresentation of pathways connected to wound healing. Tick feeding affected keratinocyte proliferation in a density-dependent manner, which relied on EVs and dendritic epidermal T cells (DETCs). This occurrence was linked to phosphoinositide 3-kinase activity, keratinocyte growth factor (KGF) and transforming growth factor β (TGF-β) levels. Collectively, we uncovered a strategy employed by a blood-feeding arthropod that impairs the integrity of the epithelial barrier, contributing to ectoparasite fitness.
Databáze: MEDLINE