Comparative oil extraction from mutt (Myliobatis goodei) liver by enzymatic hydrolysis: free versus immobilized biocatalyst.

Autor: Morales AH; Laboratorio de Biocatálisis y Biomateriales, Planta Piloto de Procesos Industriales Microbiológicos, PROIMI-CONICET, San Miguel de Tucumán, 4000, Argentina., Pisa JH; Laboratorio de Biocatálisis y Biomateriales, Planta Piloto de Procesos Industriales Microbiológicos, PROIMI-CONICET, San Miguel de Tucumán, 4000, Argentina., Gómez MI; Instituto de Química Inorgánica, Facultad de Bioquímica, Química y Farmacia, UNT, San Miguel de Tucumán, 4000, Argentina., Romero CM; Laboratorio de Biocatálisis y Biomateriales, Planta Piloto de Procesos Industriales Microbiológicos, PROIMI-CONICET, San Miguel de Tucumán, 4000, Argentina.; Instituto de Química Inorgánica, Facultad de Bioquímica, Química y Farmacia, UNT, San Miguel de Tucumán, 4000, Argentina., Vittone M; Instituto Nacional de Investigación y Desarrollo Pesquero, INIDEP, Mar del Plata, Argentina., Massa AE; Instituto Nacional de Investigación y Desarrollo Pesquero, INIDEP, Mar del Plata, Argentina.; Instituo de Investigaciones Marinas y Costeras, IIMYC, Universidad Nacional de Mar del Plata (UNMdP-CONICET), Mar del Plata, Argentina., Lamas DL; Instituto Nacional de Investigación y Desarrollo Pesquero, INIDEP, Mar del Plata, Argentina.; Instituo de Investigaciones Marinas y Costeras, IIMYC, Universidad Nacional de Mar del Plata (UNMdP-CONICET), Mar del Plata, Argentina.
Jazyk: angličtina
Zdroj: Journal of the science of food and agriculture [J Sci Food Agric] 2024 Mar 15; Vol. 104 (4), pp. 2493-2501. Date of Electronic Publication: 2023 Nov 28.
DOI: 10.1002/jsfa.13140
Abstrakt: Background: The development and fine-tuning of biotechnological processes for fish oil extraction constitute a very important focus to contribute to the development of a food industry based on fish consumption. This work lies in a comparative analysis of the oil extraction yield of Myliobatis goodei livers using free and immobilized enzymes.
Results: An immobilized biocatalyst was designed from the cell-free extract of a Bacillus sp. Mcn4. A complete factorial design was used to study the components of the bacterial culture medium and select the condition with the highest titers of extracellular enzymatic activities. Wheat bran had a significant effect on the culture medium composition for enzymatic production. The immobilized biocatalyst was designed by covalent binding of the proteins present in the cocktail retaining a percentage of different types of enzymatic activities (Mult.Enz@MgFe 2 O 4 ). Among the biocatalyst used, Alcalase® 2.4 L and Purazyme® AS 60 L (free commercial proteases) showed extraction yields of 87.39% and 84.25%, respectively, while Mult.Enz@MgFe 2 O 4 achieved a better one of 89.97%. The oils obtained did not show significant differences in their physical-chemical properties while regarding the fatty acid content, the oil extracted with Purazyme® AS 60 L showed a comparatively lower proportion of polyunsaturated fatty acids.
Conclusions: Our results suggest that the use of by-products of M. goodei is a valid alternative and encourages the use of immobilized multienzyme biocatalysts for the treatment of complex substrates in the fishing industry. © 2023 Society of Chemical Industry.
(© 2023 Society of Chemical Industry.)
Databáze: MEDLINE
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