Quantitative evaluation of warm acupuncture-moxibustion in improvement of cartilage damage in rabbits with early knee osteoarthritis based on MR T2 mapping.

Autor:	Wang ML; Department of Radiology, (, General Hospital of Ningxia Medical University, Yinchuan 750004, China). 704554548@qq.com., Li C; Department of Traditional Chinese Medicine Orthopedics and Traumatology, General Hospital of Ningxia Medical University, Yinchuan 750004, China), (, General Hospital of Ningxia Medical University, Yinchuan 750004, China., Liu JW; Department of Traditional Chinese Medicine Orthopedics and Traumatology, General Hospital of Ningxia Medical University, Yinchuan 750004, China), (, General Hospital of Ningxia Medical University, Yinchuan 750004, China., Ma XX; Department of Traditional Chinese Medicine Orthopedics and Traumatology, General Hospital of Ningxia Medical University, Yinchuan 750004, China), (, General Hospital of Ningxia Medical University, Yinchuan 750004, China., Zhang YL; Department of Traditional Chinese Medicine Orthopedics and Traumatology, General Hospital of Ningxia Medical University, Yinchuan 750004, China), (, General Hospital of Ningxia Medical University, Yinchuan 750004, China., Liu D; Department of Traditional Chinese Medicine Orthopedics and Traumatology, General Hospital of Ningxia Medical University, Yinchuan 750004, China), (, General Hospital of Ningxia Medical University, Yinchuan 750004, China. 18209583551@163.com.
Jazyk:	English; Chinese
Zdroj:	Zhen ci yan jiu = Acupuncture research [Zhen Ci Yan Jiu] 2023 Nov 25; Vol. 48 (11), pp. 1117-1124.
DOI:	10.13702/j.1000-0607.20221433
Abstrakt:	Objectives: To observe the application value of MR T2 mapping for evaluating the effect of warm acupuncture-moxibustion on articular cartilage degeneration, and to observe the relationship between T2 value and expression of matrix metalloproteinases (MMP)-1 and MMP-13 of chondrocytes in rabbits with early knee osteoarthritis (KOA). Methods: Thirty male New Zealand rabbits were randomly divided into blank control, KOA model and warm acupuncture-moxibustion groups, with 10 rabbits in each group. The early KOA model was established by right hind limb tubular plaster extension fixation method for 2 weeks. The rabbits of the warm acupuncture-moxibustion group received warm acupuncture-moxibustion stimulation at "Heding"(EX-LE2), "Neixiyan"(EX-LE4), "Waixiyan" (EX-LE5) and"Zusanli"(ST36) on the right hind limb for 15 min, once a day for 2 weeks. After intervention, MR T2 mapping of the right knee joint was performed in each group. The H.E. staining was used to evaluate the histopathological changes of cartilage, followed by giving a score according to the standards of Mankin scoring. The TUNEL method was used to analyze the apoptosis state of chondrocytes, and the positive expressions of MMP-1 and MMP-13 in the articular cartilage were detected by immunohistochemical staining. Results: Compared with the blank control group, the Mankin score, chondrocyte apoptosis index, T2 value and the positive expressions of MMP-1 and MMP-13 in the cartilage tissue were significantly increased in the model group ( P <0.01). Compared with the model group, the Mankin score, chondrocyte apoptosis index, T2 value and the positive expressions of MMP-1 and MMP-13 in the cartilage tissue were markedly decreased in the warm acupuncture-moxibustion group ( P <0.01). The T2 value was positively correlated with the expression levels of MMP-1 and MMP-13 ( P <0.01). H.E. staining showed disordered arrangement of chondrocytes and thinner cartilage layer in the model group, and a clear and relative ordered arrangement of chondrocyte in the warm acupuncture-moxibustion group. Conclusions: Warm acupuncture-moxibustion can reduce the T2 value of articular cartilage in early KOA rabbits, which is positively correlated with the decreased expression of MMP-1 and MMP-13 in the extracellular matrix of cartilage. The MR T2 mapping has certain value in evaluating the effect of warm acupuncture-moxibustion on KOA rabbits with early cartilage degeneration.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu