Loss of function mutations at the tomato SSI2 locus impair plant growth and development by altering the fatty acid desaturation pathway.

Autor: Quevedo-Colmena AS; Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, Almería, Spain., Ortiz-Atienza A; Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, Almería, Spain., Jáquez-Gutiérrez M; Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, Valencia, Spain., Quinet M; Université catholique de Louvain, Ottignies-Louvain-la-Neuve, Belgium., Atarés A; Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, Valencia, Spain., Yuste-Lisbona FJ; Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, Almería, Spain., Moreno V; Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, Valencia, Spain., Angosto T; Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, Almería, Spain., Lozano R; Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, Almería, Spain.
Jazyk: angličtina
Zdroj: Plant biology (Stuttgart, Germany) [Plant Biol (Stuttg)] 2024 Jan; Vol. 26 (1), pp. 106-116. Date of Electronic Publication: 2023 Nov 20.
DOI: 10.1111/plb.13591
Abstrakt: The stearoyl-ACP desaturase (SACPD) is a key enzyme in the regulation of saturated to unsaturated fatty acid ratio, playing a crucial role in regulating membrane stability and fluidity, as well as photosynthesis efficiency, which makes it an important research focus in crop species. This study reports the characterization and molecular cloning of pale dwarf (pad), a new tomato (Solanum lycopersicum L.) T-DNA recessive mutant, which exhibits a dwarf and chlorotic phenotype. Functional studies of the T-DNA tagged gene were conducted, including phylogenetic analysis, expression and metabolomic analyses, and generation of CRISPR/Cas9 knockout lines. The cloning of T-DNA flanking genomic sequences and a co-segregation analysis found the pad phenotype was caused by a T-DNA insertion disrupting the tomato homologue of the Arabidopsis SUPPRESSOR OF SALICYLIC ACID INSENSITIVITY 2 (SlSSI2), encoding a plastid localized isoform of SACPD. The phenotype of CRISPR/Cas9 SlSSI2 knockout lines confirmed that the morphological abnormalities in pad plants were due to SlSSI2 loss of function. Functional, metabolomic and expression analyses proved that SlSSI2 disruption causes deficiencies in 18:1 fatty acid desaturation and leads to diminished jasmonic acid (JA) content and increased salicylic acid (SA) levels. Overall, these results proved that SSI2 plays a crucial role in the regulation of polyunsaturated fatty acid profiles in tomato, and revealed that SlSSI2 loss of function results in an inhibited JA-responsive signalling pathway and a constitutively activated SA-mediated defence signalling response. This study lays the foundation for further research on tomato SACPDs and their role in plant performance and fitness.
(© 2023 The Authors. Plant Biology published by John Wiley & Sons Ltd on behalf of German Society for Plant Sciences, Royal Botanical Society of the Netherlands.)
Databáze: MEDLINE
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