The genome of a bunyavirus cannot be defined at the level of the viral particle but only at the scale of the viral population.

Autor:	Yvon M; PHIM, Univ Montpellier, INRAE, CIRAD, IRD, Institut Agro, Montpellier 34398, France., German TL; Department of Entomology, University of Wisconsin, Wisconsin 53706, Madison., Ullman DE; Department of Entomology and Nematology, University of California, California 95616, Davis., Dasgupta R; Department of Entomology, University of Wisconsin, Wisconsin 53706, Madison., Parker MH; Department of Entomology, University of Wisconsin, Wisconsin 53706, Madison., Ben-Mahmoud S; Department of Entomology and Nematology, University of California, California 95616, Davis., Verdin E; Pathologie végétale, INRAE, Avignon 84143, France., Gognalons P; Pathologie végétale, INRAE, Avignon 84143, France., Ancelin A; CBS, Univ Montpellier, CNRS, INSERM, Montpellier 34090, France., Laï Kee Him J; CBS, Univ Montpellier, CNRS, INSERM, Montpellier 34090, France., Girard J; CBS, Univ Montpellier, CNRS, INSERM, Montpellier 34090, France., Vernerey MS; PHIM, Univ Montpellier, INRAE, CIRAD, IRD, Institut Agro, Montpellier 34398, France., Fernandez E; PHIM, Univ Montpellier, INRAE, CIRAD, IRD, Institut Agro, Montpellier 34398, France., Filloux D; PHIM, Univ Montpellier, INRAE, CIRAD, IRD, Institut Agro, Montpellier 34398, France., Roumagnac P; PHIM, Univ Montpellier, INRAE, CIRAD, IRD, Institut Agro, Montpellier 34398, France., Bron P; CBS, Univ Montpellier, CNRS, INSERM, Montpellier 34090, France., Michalakis Y; MIVEGEC, Univ Montpellier, CNRS, IRD, Montpellier 34394, France., Blanc S; PHIM, Univ Montpellier, INRAE, CIRAD, IRD, Institut Agro, Montpellier 34398, France.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 Nov 28; Vol. 120 (48), pp. e2309412120. Date of Electronic Publication: 2023 Nov 20.
DOI:	10.1073/pnas.2309412120
Abstrakt:	Bunyaviruses are enveloped negative or ambisense single-stranded RNA viruses with a genome divided into several segments. The canonical view depicts each viral particle packaging one copy of each genomic segment in one polarity named the viral strand. Several opposing observations revealed nonequal ratios of the segments, uneven number of segments per virion, and even packaging of viral complementary strands. Unfortunately, these observations result from studies often addressing other questions, on distinct viral species, and not using accurate quantitative methods. Hence, what RNA segments and strands are packaged as the genome of any bunyavirus remains largely ambiguous. We addressed this issue by first investigating the virion size distribution and RNA content in populations of the tomato spotted wilt virus (TSWV) using microscopy and tomography. These revealed heterogeneity in viral particle volume and amount of RNA content, with a surprising lack of correlation between the two. Then, the ratios of all genomic segments and strands were established using RNA sequencing and qRT-PCR. Within virions, both plus and minus strands (but no mRNA) are packaged for each of the three L, M, and S segments, in reproducible nonequimolar proportions determined by those in total cell extracts. These results show that virions differ in their genomic content but together build up a highly reproducible genetic composition of the viral population. This resembles the genome formula described for multipartite viruses, with which some species of the order Bunyavirales may share some aspects of the way of life, particularly emerging properties at a supravirion scale. Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze:	MEDLINE
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