| Autor: |
Ulaangerel T; lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China., Yi M; lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China., Budsuren U; lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.; School of Animal Science and Biotechnology, Mongolian University of Life Sciences, Ulaanbaatar, Mongolia., Shen Y; lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China., Ren H; lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China., Demuul B; lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China., Bai D; lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China., Dorjgotov D; School of Industrial Technology, Mongolian University of Science and Technology, Ulaanbaatar, Mongolia., Davaakhuu G; Institute of General and Experimental Biology, Mongolian Academy of Science, Ulaanbaatar, Mongolia., Jambal T; School of Industrial Technology, Mongolian University of Science and Technology, Ulaanbaatar, Mongolia., Dugarjav M; lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China., Bou G; lnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China. |
| Abstrakt: |
Satellite cells are an important cellular model for studying muscle growth and development and mammalian locomotion-related molecular mechanisms. In this study, we investigated the effects of voltage, pulse duration, and DNA dosage on horse skeletal muscle satellite cells' electroporation transfection efficiency using the eukaryotic expression plasmid Td Tomato-C1 (5.5 kb) encoding the red fluorescent protein gene mainly based on fluorescence-positive cell rate and cell survival rate. By comparison of different voltages, pulse durations, and DNA doses, horse skeletal muscle satellite cells have nearly 80% transfection efficiency under the condition of voltage 120 V, DNA dosage 7 µg/ml, and pulse duration 30 ms. This optimized electroporation condition would facilitate the application of horse skeletal muscle satellite cells in genetic studies of muscle function and related diseases. |
