Amide-decorated reusable C 18 silica-packed columns for the rapid, efficient and sequential separation of lanthanoids using reversed phase-high performance liquid chromatography.

Autor: Kongasseri A; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu 632014, India., Madhesan T; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu 632014, India., Krishna Kumar S; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu 632014, India., Pedugu Sivaraman S; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu 632014, India., Mitra S; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu 632014, India., Kancharlapalli Chinaraga P; Indira Gandhi Centre for Atomic Research, Homi Bhabha National Institute (HBNI), Kalpakkam, Tamil Nadu 603102, India., Rao CVSB; Indira Gandhi Centre for Atomic Research, Homi Bhabha National Institute (HBNI), Kalpakkam, Tamil Nadu 603102, India., Nagarajan S; Indira Gandhi Centre for Atomic Research, Homi Bhabha National Institute (HBNI), Kalpakkam, Tamil Nadu 603102, India., Deivasigamani P; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu 632014, India. Electronic address: prabhakaran.d@vit.ac.in., Mohan AM; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu 632014, India. Electronic address: akhila.maheswari@vit.ac.in.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2024 Jan 04; Vol. 1713, pp. 464509. Date of Electronic Publication: 2023 Nov 14.
DOI: 10.1016/j.chroma.2023.464509
Abstrakt: The current work focuses on the sequential separation of trivalent lanthanides (except Pm 3+ ) using modified C 18 silica-packed supports through the reversed-phase high-performance liquid chromatography (RP-HPLC) technique. In the current research, four indigenously synthesized amphiphilic aromatic triamide derivatives, namely N 1 , N 1 , N 3 , N 3 , N 5 , N 5 -hexa(alkyl) benzene-1,3,5-tri carboxamide (alkyl = butyl, hexyl, octyl, and decyl), were employed as column modifiers. The results show that the separation of Ln 3+ can be achieved systematically (< 12 min) by tuning the modifiers' functional group and hydrophobic chain and fine-tuning the column modification procedure and separation parameters. The chromatographic studies revealed that the use of 0.168 mmol of N 1 , N 1 , N 3 , N 3 ,N 5 , N 5 -hexa(hexyl)benzene-1,3,5-tricarboxamide (HHBTA) coated column and 0.419 mmol of N 1 , N 1 , N 3 , N 3 , N 5 , N 5 -hexa(octyl) benzene-1,3,5-tricarboxamide (HOBTA) modified columns offered excellent separation for the lanthanoids, using 0.1 M α-hydroxyisobutyric acid (HIBA), as mobile phase. The separated lanthanoids were quantified by post-column derivatization reaction (after the separation process) using Arsenazo (III) as the post-column reagent by integrating with a UV-Visible detector fixed at 655 nm (λ max ). A systematic study on the influence of various analytical features, such as the effect of the modifier's chain length and its concentration, mobile phase composition and pH, was performed and optimized for achieving the best separation protocols.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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