Development of enantioselective high-performance liquid chromatography-tandem mass spectrometry method for the quantitative determination of 3,4-methylenedioxy-methamphetamine (MDMA) and its phase-1 metabolites in human biological fluids.

Autor: Lo Faro AF; Department of Excellence-Biomedical Sciences and Public Health, Università Politecnica delle Marche, 60121 Ancona, Italy., Sprega G; Department of Excellence-Biomedical Sciences and Public Health, Università Politecnica delle Marche, 60121 Ancona, Italy., Beradinelli D; Department of Excellence-Biomedical Sciences and Public Health, Università Politecnica delle Marche, 60121 Ancona, Italy., Tini A; Department of Excellence-Biomedical Sciences and Public Health, Università Politecnica delle Marche, 60121 Ancona, Italy., Poyatos L; Servei de Farmacologia Clínica, Hospital Universitari Germans Trias i Pujol (HUGTiP, IGTP)- Universitat Aut`onoma de Barcelona, Unitat Docent HUGTiP, Badalona, Spain., Papaseit E; Servei de Farmacologia Clínica, Hospital Universitari Germans Trias i Pujol (HUGTiP, IGTP)- Universitat Aut`onoma de Barcelona, Unitat Docent HUGTiP, Badalona, Spain., Berretta P; National Centre on Addiction and Doping, Istituto Superiore di Sanit`a, Rome, Italy., Di Giorgi A; National Centre on Addiction and Doping, Istituto Superiore di Sanit`a, Rome, Italy., Farre M; Servei de Farmacologia Clínica, Hospital Universitari Germans Trias i Pujol (HUGTiP, IGTP)- Universitat Aut`onoma de Barcelona, Unitat Docent HUGTiP, Badalona, Spain., Takaishvili N; Tbilisi State University, Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, 0179 Tbilisi, Georgia., Farkas T; Phenomenex Inc., 411 Madrid Ave., Torrance 90501, CA, USA., Busardò FP; Department of Excellence-Biomedical Sciences and Public Health, Università Politecnica delle Marche, 60121 Ancona, Italy. Electronic address: f.p.busardo@univpm.it., Chankvetadze B; Tbilisi State University, Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, 0179 Tbilisi, Georgia.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2024 Jan 20; Vol. 238, pp. 115768. Date of Electronic Publication: 2023 Oct 05.
DOI: 10.1016/j.jpba.2023.115768
Abstrakt: In the present study enantioselective high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods were developed for the quantitative determination of 3,4-methylenedioxy-methamphetamine (MDMA) and its major phase-1 metabolites 4-hydroxy-3-methoxyamphetamine (HMA), 4-hydroxy-3-methoxymethamphetamine (HMMA) and 3,4-methylenedioxyamphetamine (MDA) in human plasma, sweat, oral fluid (OF) and urine. The simultaneous separation of all these compounds and their respective enantioseparation was accomplished on two polysaccharide-based chiral columns. The Lux AMP column with a proprietary chiral selector enabled baseline separation of the enantiomers of MDMA, HMA and HMMA while MDA enantiomers could not be separated with this column under the experimental conditions used in this study. The Lux i-Amylose-3 column based on amylose tris(5-chloro-3-methylphenylcarbamate) as chiral selector baseline-separated the enantiomers of MDMA, HMMA and MDA while the enantiomers of HMA could not be separated. Thus, the various samples were analyzed by using both columns alternatively in combinations with acetonitrile containing 25% (v/v) 5 mM ammonium bicarbonate buffer at pH 11.0 as mobile phase. Analysis time was less than 4 min with the Lux AMP column and less than 6 min with the Lux i-Amylose-3 column. Both methods were validated and applied to the enantioselective determination of MDMA and its phase-I metabolites in human biological fluids, and enantioselective metabolism of MDMA was confirmed.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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