Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery.

Autor:	Zhang N; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA., Guo Y; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA., Foiret J; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA., Tumbale SK; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA., Paulmurugan R; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA., Ferrara KW; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA. Electronic address: kwferrar@stanford.edu.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102723. Date of Electronic Publication: 2023 Nov 16.
DOI:	10.1016/j.xpro.2023.102723
Abstrakt:	Microbubbles are currently approved for diagnostic ultrasound imaging and are under evaluation in therapeutic protocols. Here, we present a protocol for in vitro sonoporation validation using non-targeted microbubbles for gene delivery. We describe steps for computational simulation, experimental calibration, reagent preparation, ultrasound treatment, validation, and gene expression analysis. This protocol uses approved diagnostic microbubbles and parameters that are applicable for human use. For complete details on the use and execution of this protocol, please refer to Bez et al. (2017). 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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