Melatonin and probiotic administration ameliorated hyperglycaemia, oxidative stress, and enhanced cytoprotective effect on beta-cells of diabetic rats.

Autor: Aluwong T; Department of Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria., Sumanu VO; Department of Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria., Abdulsalam RA; Department of Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria., Emmanuel DS; Department of Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria., Ezekiel NG; Department of Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria., Aliyu MB; Department of Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria., Ayo JO; Department of Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria., Ukwenu JO; Department of Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria., Yaro JD; Department of Pathology, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria., Ogbuagu NE; Department of Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria.
Jazyk: angličtina
Zdroj: Journal of diabetes and metabolic disorders [J Diabetes Metab Disord] 2023 Sep 05; Vol. 22 (2), pp. 1537-1549. Date of Electronic Publication: 2023 Sep 05 (Print Publication: 2023).
DOI: 10.1007/s40200-023-01284-4
Abstrakt: Objective: The study aimed at investigating the effects of administering melatonin and a probiotic to streptozotocin-induced diabetic rats on hyperglycaemia, oxidative stress biomarkers and beta-cells.
Design: Type 1 diabetes was induced in 5 months-old male Wistar rats by single intraperitoneal (i.p.) administration of freshly-prepared STZ (60 mg/kg body weight). Six groups of 10 rats were used and treated once daily for six weeks; (1) Healthy control: normal saline only; (2) Pre-treated with Melatonin (MEL); (3) Diabetic control; (4) Diabetic + Treated with MEL; (5) Diabetic + Treated with Probiotic (Prob); (6) Diabetic + Treated with MEL + Prob. Blood glucose, body weight, activities of antioxidant enzymes and malondialdehyde concentration in serum and tissues, reduced glutathione and immunohistochemical assay. Data obtained were expressed as mean ± standard error of the mean (Mean ± SEM) and subjected to ANOVA followed by Tukey's post hoc test.
Results: Melatonin + Probiotic significantly decreased blood glucose concentrations in diabetic treated rats, compared to the diabetic control rats. MEL + Probiotic increased (p < 0.05) superoxide dismutase activity in serum and liver of diabetic rats. MEL + Probiotic reduced (p < 0.05) malondialdehyde concentration in the serum, liver and kidneys, respectively. MEL + Probiotic treated diabetic rats displayed islets with much greater content of insulin.
Conclusion: Melatonin + Probiotic combination was more effective in mitigating hyperglycaemia, oxidative stress, and exerted cytoprotective effect on the beta-cells.
Competing Interests: Conflict of interestThe authors declare no conflict of interest.
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Databáze: MEDLINE
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