Stimulator of interferon genes/Interferon regulatory factor 3 (STING-IRF3) and inflammasome-activation mediated pyroptosis biomarkers: a network of integrated pathways in diabetic nephropathy.

Autor: El-Deeb OS; Medical Biochemistry Department, Faculty of Medicine, Tanta University, El Geesh Street, Tanta, 31511 Egypt., Hafez YM; Internal Medicine Department, Faculty of Medicine, Tanta University, Tanta, Egypt., Eltokhy AK; Medical Biochemistry Department, Faculty of Medicine, Tanta University, El Geesh Street, Tanta, 31511 Egypt., Awad MM; Physiology Department, Faculty of Medicine, Tanta University, Tanta, Egypt., El-Shaer RAA; Physiology Department, Faculty of Medicine, Tanta University, Tanta, Egypt., Abdel Ghafar MT; Clinical Pathology Department, Faculty of Medicine, Tanta University, Tanta, Egypt., Atef MM; Medical Biochemistry Department, Faculty of Medicine, Tanta University, El Geesh Street, Tanta, 31511 Egypt.
Jazyk: angličtina
Zdroj: Journal of diabetes and metabolic disorders [J Diabetes Metab Disord] 2023 Aug 09; Vol. 22 (2), pp. 1471-1480. Date of Electronic Publication: 2023 Aug 09 (Print Publication: 2023).
DOI: 10.1007/s40200-023-01270-w
Abstrakt: Background: Diabetic Nephropathy (DN) is serious diabetic complication affecting the structure and function of the kidney. This study assessed the stimulator of interferon genes/ Interferon regulatory factor 3 (STING/IRF3) signaling pathway roles and inflammasome-activation mediated pyroptosis, being imperative pathways of inordinate importance in disease progression, in DN throughout its different stages.
Methods: 45 Diabetic cases were categorized into three groups based on their albuminuric status as follow: Normoalbuminuric, Microalbuminuric and Macroalbuminuric diabetic groups and 15 healthy subjects as controls were included. We evaluated STING and absent in melanoma 2 ( AIM2 ) messenger RNA (mRNA) expressions from whole blood using quantitative RT-PCR. Additionally, Serum levels of STING, AIM2, IRF3, Nod like receptor pyrins-3 (NLRP3), interleukin-1β (IL-1β) and caspase-1 were assessed by ELISA technique.
Results: The study documented that STING and AIM2 mRNA expressions had significantly increased in DN cases with highest value in macroalbuminuric diabetic groups ( p  < 0.001*). Parallel results were observed concerning serum STING, AIM2, IRF3, NLRP3, Caspase-1 in addition to IL-1β levels ( p  < 0.001*).
Conclusion: The study documented the forthcoming role of STING in DN progression and its positive correlation with inflammasome-activation mediated pyroptosis biomarkers throughout its three different stages; launching new horizons in DN pathogenesis by highlighting its role as a reliable prognostic biomarker.
Competing Interests: Conflict of interestNo competing interests.
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Databáze: MEDLINE
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