Micro-ultrasound based characterization of cerebrovasculature following focal ischemic stroke and upon short-term rehabilitation.

Autor:	Yu J; Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.; Physical Sciences, Sunnybrook Research Institute, Toronto, Ontario, Canada., Joo IL; Physical Sciences, Sunnybrook Research Institute, Toronto, Ontario, Canada., Bazzigaluppi P; Physical Sciences, Sunnybrook Research Institute, Toronto, Ontario, Canada.; MetaCell, Cagliari, Italy., Koletar MM; Physical Sciences, Sunnybrook Research Institute, Toronto, Ontario, Canada., Cherin E; Physical Sciences, Sunnybrook Research Institute, Toronto, Ontario, Canada., Stanisz AG; Physical Sciences, Sunnybrook Research Institute, Toronto, Ontario, Canada., Graham JW; Physical Sciences, Sunnybrook Research Institute, Toronto, Ontario, Canada., Demore C; Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.; Physical Sciences, Sunnybrook Research Institute, Toronto, Ontario, Canada., Stefanovic B; Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.; Physical Sciences, Sunnybrook Research Institute, Toronto, Ontario, Canada.
Jazyk:	angličtina
Zdroj:	Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism [J Cereb Blood Flow Metab] 2024 Apr; Vol. 44 (4), pp. 461-476. Date of Electronic Publication: 2023 Nov 16.
DOI:	10.1177/0271678X231215004
Abstrakt:	Notwithstanding recanalization treatments in the acute stage of stroke, many survivors suffer long-term impairments. Physical rehabilitation is the only widely available strategy for chronic-stage recovery, but its optimization is hindered by limited understanding of its effects on brain structure and function. Using micro-ultrasound, behavioral testing, and electrophysiology, we investigated the impact of skilled reaching rehabilitation on cerebral hemodynamics, motor function, and neuronal activity in a rat model of focal ischemic stroke. A 50 MHz micro-ultrasound transducer and intracortical electrophysiology were utilized to characterize neurovascular changes three weeks following focal ischemia elicited by endothelin-1 injection into the sensorimotor cortex. Sprague-Dawley rats were rehabilitated through tray reaching, and their fine skilled reaching was assessed via the Montoya staircase. Focal ischemia led to a sustained deficit in forelimb reaching; and increased tortuosity of the penetrating vessels in the perilesional cortex; with no lateralization of spontaneous neuronal activity. Rehabilitation improved skilled reaching; decreased cortical vascularity; was associated with elevated peri- vs. contralesional hypercapnia-induced flow homogenization and increased perilesional spontaneous cortical neuronal activity. Our study demonstrated neurovascular plasticity accompanying rehabilitation-elicited functional recovery in the subacute stage following stroke, and multiple micro-ultrasound-based markers of cerebrovascular structure and function modified in recovery from ischemia and upon rehabilitation. Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze:	MEDLINE
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