Could alder buckthorn (Frangula alnus Mill) be a source of chemotherapeutics effective against hepato- and colorectal carcinoma? An in vitro study.

Autor: Vuletić S; University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade, Serbia. Electronic address: stefana.d@bio.bg.ac.rs., Bekić M; University of Belgrade, Institute for the Application of Nuclear Energy, INEP, Banatska 31b, 11080 Belgrade, Serbia., Tomić S; University of Belgrade, Institute for the Application of Nuclear Energy, INEP, Banatska 31b, 11080 Belgrade, Serbia., Nikolić B; University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade, Serbia., Cvetković S; University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade, Serbia., Ganić T; University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade, Serbia., Mitić-Ćulafić D; University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade, Serbia.
Jazyk: angličtina
Zdroj: Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2023 Nov-Dec; Vol. 892, pp. 503706. Date of Electronic Publication: 2023 Oct 14.
DOI: 10.1016/j.mrgentox.2023.503706
Abstrakt: Among numerous types of cancer, hepatocellular and colorectal carcinoma are important causes of mortality. Given the nature of these cancer types and their resistance, it is of great importance to find new chemotherapeutics and therapy targets, so plant products seem to be an excellent choice in such search. The main goal of this study was to investigate anticancer activity of Frangula alnus ethyl-acetate extract (FA) and its dominant constituent emodin (E) on hepatocellular and colorectal carcinoma cell lines, HepG2 and HCT116, as well as on normal MRC-5 fibroblasts. Cytotoxicity was investigated in MTT test and both FA and E showed strong reduction of cell viability in cancer cells. Flow cytometer analysis demonstrated that FA and E led to G1 phase arrest and slight accumulation of cells in the G2/M phase; additionally, annexinV-FITC/7AAD dying showed that FA and E decreased cell viability and triggered apoptosis in all cell lines. FA and E evidenced strong genotoxic potential in comet assay performed on all cell lines, while tests measuring antioxidative potential (DPPH and TBA) demonstrated strong effect of FA. It could be concluded that both FA and E have significant anticancer activity against hepatocellular and colorectal carcinoma cell lines HepG2 and HCT116, but notable selectivity was not observed.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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