Decreased DNA repair capacity caused by exposure to metal mixtures is modulated by the PARP1 rs1136410 variant in newborns from a polluted metropolitan area.

Autor: Paz-Sabillón M; Department of Toxicology, Cinvestav, Ave. IPN 2508, San Pedro Zacatenco, Mexico City, 07360, Mexico., Montes-Castro N; Department of Health Sciences, Autonomous University of the West, Culiacán Regional Unit, Lola Beltrán Blvd, Culiacán, Sinaloa, 80020, Mexico., Torres-Sánchez L; Center for Population Health Research, National Institute of Public Health, Ave. Universidad 655, Santa María Ahuacatitlán, Cuernavaca, Morelos, 62100, Mexico., Del Razo LM; Department of Toxicology, Cinvestav, Ave. IPN 2508, San Pedro Zacatenco, Mexico City, 07360, Mexico., Córdova EJ; Oncogenomics Consortium Laboratory, National Institute of Genomic Medicine, Department of Clinic Research, Arenal Tepepan, Mexico City, 14610, Mexico., Quintanilla-Vega B; Department of Toxicology, Cinvestav, Ave. IPN 2508, San Pedro Zacatenco, Mexico City, 07360, Mexico. Electronic address: mquintan@cinvestav.mx.
Jazyk: angličtina
Zdroj: Environmental research [Environ Res] 2024 Jan 15; Vol. 241, pp. 117631. Date of Electronic Publication: 2023 Nov 14.
DOI: 10.1016/j.envres.2023.117631
Abstrakt: Background: DNA damage caused by exposure to metal mixtures and the potential modulating role of genes involved in DNA repair and the antioxidant response have not been evaluated in newborns.
Aim: The aim was to evaluate the association between prenatal exposure to metal mixtures and DNA repair capacity (DRC) in newborns from the Metropolitan Area of Mexico City (MAMC), a heavily polluted area, and the impact of variants in genes involved in DNA repair and the antioxidant response on this association.
Methods: We analyzed cord blood samples obtained at delivery from 125 healthy newborns from the MAMC. Twenty-four elements were determined by inductively coupled plasma mass spectrometry (ICP‒MS), but only 12 (Cu, I, Se, Zn, As, Ba, Cs, Mn, Sb, Sr, Pb, and Ti) were quantified in most samples. DRC was assessed by the challenge-comet assay, and OGG1, PARP1, and NFE2L2 genotyping was performed with TaqMan probes. Metal mixtures were identified and analyzed using principal component analysis (PCA) and weighted quantile sum (WQS) regression. Independent adjusted linear regression models were used to evaluate the associations.
Results: A null DRC was observed in 46% of newborns. The metals with the highest concentrations were Mn, Sr, Ti, and Pb. Essential elements showed normal levels. Only the mixture characterized by increased As, Cs, Cu, Se, and Zn levels was inversely associated with DRC. As was the principal contributor (37.8%) in the negative direction in the DRC followed by Ba and Sb, according to the WQS regression. Newborns carrying of the derived (G) allele of the PARP1 rs1136410 variant showed decreased DRC by exposure to some potentially toxic metals (PTMs) (As, Cs, and Ba).
Conclusion: Prenatal exposure to metal mixtures negatively affected DRC in newborns, and the PARP1 rs1136410 variant had a modulating role in this association.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: