Islet architecture in adult mice is actively maintained by Robo2 expression in β cells.

Autor: Waters BJ; Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI, 53705, USA., Birman ZR; Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI, 53705, USA., Wagner MR; Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI, 53705, USA., Lemanski J; Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI, 53705, USA., Blum B; Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI, 53705, USA. Electronic address: bblum4@wisc.edu.
Jazyk: angličtina
Zdroj: Developmental biology [Dev Biol] 2024 Jan; Vol. 505, pp. 122-129. Date of Electronic Publication: 2023 Nov 15.
DOI: 10.1016/j.ydbio.2023.11.003
Abstrakt: A fundamental question in developmental biology is whether tissue architectures formed during development are set for life, or require continuous maintenance signals, and if so, what are those signals. The islets of Langerhans in the pancreas can serve as an elegant model tissue to answer these questions. Islets have a non-random spatial architecture, which is important to proper glucose homeostasis. Islet architecture forms during embryonic development, in a morphogenesis process partially involving expression of Roundabout (Robo) receptors in β cells, and their ligand, Slit, in the surrounding mesenchyme. Whether islet architecture is set during development and remains passive in adulthood, or whether it requires active maintenance throughout life, has not been determined. Here we conditionally deleted Robo2 in β cells of adult mice and observed their islet architecture following a two-month chase. We show that deleting Robo2 in adult β cells causes significant loss of islet architecture without affecting β cell identity, maturation, or stress, indicating that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required to maintain islet architecture, and thus optimize islet function, is important for developing future diabetes therapies.
Competing Interests: Declaration of competing interest The authors declare no conflicts of interests in relation to this work.
(Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: