The effects of morin and methotrexate on pentose phosphate pathway enzymes and GR/GST/TrxR enzyme activities: An in vivo and in silico study.

Autor: Caglayan C; Department of Medical Biochemistry, Faculty of Medicine, Bilecik Şeyh Edebali University, Bilecik, Turkey., Temel Y; Department of Solhan School of Health Services, Bingol University, Bingol, Turkey., Türkeş C; Department of Biochemistry, Faculty of Pharmacy, Erzincan Binali Yıldırım University, Erzincan, Turkey., Ayna A; Department of Chemistry, Faculty of Sciences and Arts, Bingol University, Bingol, Turkey., Ece A; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Biruni University, İstanbul, Turkey., Beydemir Ş; Department of Biochemistry, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey.; Bilecik Şeyh Edebali University, Bilecik, Turkey.
Jazyk: angličtina
Zdroj: Archiv der Pharmazie [Arch Pharm (Weinheim)] 2024 Feb; Vol. 357 (2), pp. e2300497. Date of Electronic Publication: 2023 Nov 16.
DOI: 10.1002/ardp.202300497
Abstrakt: In this study, the mechanisms by which the enzymes glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), glutathione reductase (GR), glutathione-S-transferase (GST), and thioredoxin reductase (TrxR) are inhibited by methotrexate (MTX) were investigated, as well as whether the antioxidant morin can mitigate or prevent these adverse effects in vivo and in silico. For 10 days, rats received oral doses of morin (50 and 100 mg/kg body weight). On the fifth day, a single intraperitoneal injection of MTX (20 mg/kg body weight) was administered to generate toxicity. Decreased activities of G6PD, 6PGD, GR, GST, and TrxR were associated with MTX-related toxicity while morin treatment increased the activity of the enzymes. The docking analysis indicated that H-bonds, pi-pi stacking, and pi-cation interactions were the dominant interactions in these enzyme-binding pockets. Furthermore, the docked poses of morin and MTX against GST were subjected to molecular dynamic simulations for 200 ns, to assess the stability of both complexes and also to predict key amino acid residues in the binding pockets throughout the simulation. The results of this study suggest that morin may be a viable means of alleviating the enzyme activities of important regulatory enzymes against MTX-induced toxicity.
(© 2023 Deutsche Pharmazeutische Gesellschaft.)
Databáze: MEDLINE
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