Protein aggregates act as a deterministic disruptor during bacterial cell size homeostasis.

Autor: Mortier J; Department of Microbial and Molecular Systems, KU Leuven, Leuven, Belgium., Govers SK; Department of Microbial and Molecular Systems, KU Leuven, Leuven, Belgium.; Department of Biology, KU Leuven, Leuven, Belgium., Cambré A; Department of Microbial and Molecular Systems, KU Leuven, Leuven, Belgium., Van Eyken R; Department of Microbial and Molecular Systems, KU Leuven, Leuven, Belgium., Verheul J; Swammerdam Institute for Life Sciences, Bacterial Cell Biology and Physiology, University of Amsterdam, Amsterdam, The Netherlands., den Blaauwen T; Swammerdam Institute for Life Sciences, Bacterial Cell Biology and Physiology, University of Amsterdam, Amsterdam, The Netherlands., Aertsen A; Department of Microbial and Molecular Systems, KU Leuven, Leuven, Belgium. abram.aertsen@kuleuven.be.
Jazyk: angličtina
Zdroj: Cellular and molecular life sciences : CMLS [Cell Mol Life Sci] 2023 Nov 16; Vol. 80 (12), pp. 360. Date of Electronic Publication: 2023 Nov 16.
DOI: 10.1007/s00018-023-05002-4
Abstrakt: Mechanisms underlying deviant cell size fluctuations among clonal bacterial siblings are generally considered to be cryptic and stochastic in nature. However, by scrutinizing heat-stressed populations of the model bacterium Escherichia coli, we uncovered the existence of a deterministic asymmetry in cell division that is caused by the presence of intracellular protein aggregates (PAs). While these structures typically locate at the cell pole and segregate asymmetrically among daughter cells, we now show that the presence of a polar PA consistently causes a more distal off-center positioning of the FtsZ division septum. The resulting increased length of PA-inheriting siblings persists over multiple generations and could be observed in both E. coli and Bacillus subtilis populations. Closer investigation suggests that a PA can physically perturb the nucleoid structure, which subsequently leads to asymmetric septation.
(© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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