Biofilm dysbiosis and caries activity: a surface or an individual issue?

Autor:	Ev LD; Universidade Federal do Rio Grande do Sul, Faculdade de Odontologia, Departamento de Odontologia Preventiva e Social, Porto Alegre, RS, Brasil., Poloni JF; Instituto Nacional de Ciência e Tecnologia - Ciências Forense, Pontifica Universidade Católica do Rio Grande do Sul, Escola de Ciências da Saúde e da Vida, Porto Alegre, Brasil., Damé-Teixeira N; Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Odontologia, Brasília, DF, Brasil., Arthur RA; Universidade Federal do Rio Grande do Sul, Faculdade de Odontologia, Departamento de Odontologia Preventiva e Social, Porto Alegre, RS, Brasil., Corralo DJ; Universidade de Passo Fundo, Escola de Odontologia, Departamento de Odontologia, RS, Passo Fundo, Brasil., Henz SL; Universidade Federal do Rio Grande do Sul, Faculdade de Odontologia, Departamento de Odontologia Preventiva e Social, Porto Alegre, RS, Brasil., DO T; University of Leeds, School of Dentistry, Division of Oral Biology, Leeds, UK., Maltz M; Universidade Federal do Rio Grande do Sul, Faculdade de Odontologia, Departamento de Odontologia Preventiva e Social, Porto Alegre, RS, Brasil., Parolo CCF; Universidade Federal do Rio Grande do Sul, Faculdade de Odontologia, Departamento de Odontologia Preventiva e Social, Porto Alegre, RS, Brasil.
Jazyk:	angličtina
Zdroj:	Journal of applied oral science : revista FOB [J Appl Oral Sci] 2023 Nov 13; Vol. 31, pp. e20230214. Date of Electronic Publication: 2023 Nov 13 (Print Publication: 2023).
DOI:	10.1590/1678-7757-2023-0214
Abstrakt:	Objective: This study aimed to analyze the functional profile of supragingival biofilm from sound (CAs), active (CAa), and inactive (CAi) enamel caries lesions from caries-active individuals to provide insights into the diversity of biological processes regarding biofilm dysbiosis. Methodology: A metatranscriptome analysis was performed in biofilm samples collected from five caries-active individuals. Total RNA was extracted, and the microbial cDNAs were obtained and sequenced (Illumina HiSeq3000). Trimmed data were submitted to the SqueezeMeta pipeline in the co-assembly mode for functional analysis and further differential gene expression analysis (DESeq2). Results: Bioinformatics analysis of mRNAs revealed a similar functional profile related to all analyzed conditions (CAa, CAi, and CAs). However, active and inactive surfaces share up-regulated genes (gtsA; qrtT; tqsA; pimB; EPHX1) related to virulence traits that were not overrepresented in sound surfaces. From a functional perspective, what matters most is the individual carious status rather than the surface condition. Therefore, pooling samples from various sites can be carried out using naturally developed oral biofilms but should preferably include carious surfaces. Conclusion: Metatranscriptome data from subjects with caries activity have shown that biofilms from sound, arrested, and active lesions are similar in composition and function.
Databáze:	MEDLINE
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