Galvanic Displacement Reaction Enabled Specific and Sensitive Detection of Bacteria with a Digital Photocorrosion GaAs/AlGaAs Biosensor.

Autor: Singh A; Laboratory for Quantum Semiconductors and Photon-Based BioNanotechnology, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Department of Electrical and Computer Engineering, Université de Sherbrooke, 3000 boul. de l'Université, Sherbrooke, Québec J1K 0A5, Canada., Hassen WM; Laboratory for Quantum Semiconductors and Photon-Based BioNanotechnology, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Department of Electrical and Computer Engineering, Université de Sherbrooke, 3000 boul. de l'Université, Sherbrooke, Québec J1K 0A5, Canada., St-Onge R; Laboratory for Quantum Semiconductors and Photon-Based BioNanotechnology, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Department of Electrical and Computer Engineering, Université de Sherbrooke, 3000 boul. de l'Université, Sherbrooke, Québec J1K 0A5, Canada., Dubowski JJ; Laboratory for Quantum Semiconductors and Photon-Based BioNanotechnology, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Department of Electrical and Computer Engineering, Université de Sherbrooke, 3000 boul. de l'Université, Sherbrooke, Québec J1K 0A5, Canada.
Jazyk: angličtina
Zdroj: The journal of physical chemistry. C, Nanomaterials and interfaces [J Phys Chem C Nanomater Interfaces] 2023 Oct 29; Vol. 127 (44), pp. 21768-21776. Date of Electronic Publication: 2023 Oct 29 (Print Publication: 2023).
DOI: 10.1021/acs.jpcc.3c05200
Abstrakt: The conjugation of ionic gold with bacterial antibodies makes it possible to induce a specific interaction between targeted bacteria and the surface of a GaAs/AlGaAs biochip. The process of immobilization is based on a galvanic displacement reaction (GDR) involving electron transfer between GaAs and Au 3+ ions that leads to the formation of a Au-Ga alloy anchoring bacteria to the biochip surface. The GDR-based immobilization of Escherichia coli on biochips comprising a stack of GaAs/AlGaAs nanolayers ( d GaAs = 12 nm, d AlGaAs = 10 nm) was confirmed by X-ray photoelectron spectroscopy and atomic force microscopy-based infrared experiments. We report the successful application of this approach for highly sensitive detection of E. coli with a digital photocorrosion (DIP) biosensor. The photoluminescence (PL) monitored DIP of GaAs/AlGaAs nanolayers results in the formation of a PL intensity maximum whose temporal appearance depends on the electric charge transfer between bacteria and the biochip. The formation of a robust bacteria-biochip interface achieved with the GDR process allowed us to observe the role of bacteria on the temporal position of a PL intensity maximum related to the etching of two pairs of GaAs/AlGaAs nanolayers extending up to 24 nm below the biochip surface. We demonstrate the attractive detection of E. coli at 250 CFU/mL, and we discuss the potential of this approach for designing a family of biosensors addressing the quasi-continuous monitoring of a water environment for the presence of pathogenic bacteria.
Competing Interests: The authors declare no competing financial interest.
(© 2023 The Authors. Published by American Chemical Society.)
Databáze: MEDLINE
Externí odkaz: