Bee gomogenat enhances the healing process of diabetic wounds by orchestrating the connexin-pannexin gap junction proteins in streptozotocin-induced diabetic mice.

Autor: Sayed LH; Zoology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt.; Laboratory of Immunology, Zoology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt., Badr G; Zoology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt. badr73@yahoo.com.; Laboratory of Immunology, Zoology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt. badr73@yahoo.com., Omar HEM; Zoology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt., Elghaffar SKA; Pathology and Clinical Pathology Department, Faculty of Veterinary Medicine, Assiut University, Assiut, 71516, Egypt.; School of Veterinary Medicine, Badr University, Assiut, Egypt., Sayed A; Mallawi Specialized Hospital, 26Th of July Street, Mallawi, Minia, Egypt.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Nov 15; Vol. 13 (1), pp. 19961. Date of Electronic Publication: 2023 Nov 15.
DOI: 10.1038/s41598-023-47206-5
Abstrakt: Delay in wound healing remains one of diabetes's worse side effects, which increases mortality. The proposed study sought to scrutinize the implications of bee gomogenat (BG) on diabetic's wound closure in a streptozotocin-(STZ)-enhanced type-1 diabetes model's rodents. We used 3 different mice groups: group 1 non-diabetic rodents "serving as control", group 2 diabetic rodents, and group3 BG-treated diabetic rodents. We noticed that diabetic rodents experience a delayed wound closure, which emerged as a significant (*P < 0.05) decline in the deposition of collagen as compared to control non-diabetic animals. We noticed that diabetic rodents have a delayed wound closure characterized by a significant (*P < 0.05) decrease in the CD31 expression (indicator for wound angiogenesis and neovascularization) and an apparent elevation in the expression of such markers of inflammation as MCP-1 and HSP-70 as compared to control animals. Moreover, diabetic animals displayed a significant (*P < 0.05) increase in the expression of gap junction proteins Cx43 and a significant decrease in the expression of Panx3 in the wounded skin tissues when compared to the controls. Intriguingly, topical application with BG on the diabetic wounded skin tissues contributes to a significant ( # P < 0.05) enhancing in the collagen deposition, up-regulating the level of CD31 expression and a significant ( # P < 0.05) down-regulation in the MCP-1 and HSP-70 expressions as compared to diabetic non-treated animals. The expression's levels of Cx43 and Panx3 were significantly ( # P < 0.05) retrieved in diabetic rodents after BG treatment. Taken together, our findings showed for the first time that BG promotes the recovering process and accelerated the closure of diabetic related wounds.
(© 2023. The Author(s).)
Databáze: MEDLINE
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