Engineering memory with an extrinsically disordered kinase.

Autor: Ripoli C; Department of Neuroscience, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.; Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy., Dagliyan O; Division of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, 17165 Stockholm, Sweden., Renna P; Department of Neuroscience, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.; Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy., Pastore F; Department of Neuroscience, Università Cattolica del Sacro Cuore, 00168 Rome, Italy., Paciello F; Department of Neuroscience, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.; Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy., Sollazzo R; Department of Neuroscience, Università Cattolica del Sacro Cuore, 00168 Rome, Italy., Rinaudo M; Department of Neuroscience, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.; Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy., Battistoni M; Department of Neuroscience, Università Cattolica del Sacro Cuore, 00168 Rome, Italy., Martini S; Department of Neuroscience, Università Cattolica del Sacro Cuore, 00168 Rome, Italy., Tramutola A; Department of Biochemical Sciences 'A. Rossi-Fanelli', Sapienza University of Rome, 00185 Rome, Italy., Sattin A; Optical Approaches to Brain Function Laboratory, Istituto Italiano di Tecnologia, 16163 Genoa, Italy., Barone E; Department of Biochemical Sciences 'A. Rossi-Fanelli', Sapienza University of Rome, 00185 Rome, Italy., Saneyoshi T; Department of Pharmacology, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan., Fellin T; Optical Approaches to Brain Function Laboratory, Istituto Italiano di Tecnologia, 16163 Genoa, Italy., Hayashi Y; Department of Pharmacology, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan., Grassi C; Department of Neuroscience, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.; Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy.
Jazyk: angličtina
Zdroj: Science advances [Sci Adv] 2023 Nov 17; Vol. 9 (46), pp. eadh1110. Date of Electronic Publication: 2023 Nov 15.
DOI: 10.1126/sciadv.adh1110
Abstrakt: Synaptic plasticity plays a crucial role in memory formation by regulating the communication between neurons. Although actin polymerization has been linked to synaptic plasticity and dendritic spine stability, the causal link between actin polymerization and memory encoding has not been identified yet. It is not clear whether actin polymerization and structural changes in dendritic spines are a driver or a consequence of learning and memory. Using an extrinsically disordered form of the protein kinase LIMK1, which rapidly and precisely acts on ADF/cofilin, a direct modifier of actin, we induced long-term enlargement of dendritic spines and enhancement of synaptic transmission in the hippocampus on command. The activation of extrinsically disordered LIMK1 in vivo improved memory encoding and slowed cognitive decline in aged mice exhibiting reduced cofilin phosphorylation. The engineered memory by an extrinsically disordered LIMK1 supports a direct causal link between actin-mediated synaptic transmission and memory.
Databáze: MEDLINE
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