Chronic cranial window implantation for high-resolution intravital imaging of the endothelial glycocalyx in mouse cortex.

Autor:	Gray AL; Wellcome Centre for Cell-Matrix Research, Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester M13 9PT, UK; Geoffrey Jefferson Brain Research Centre, The Manchester Academic Health Science Centre, Northern Care Alliance National Health Service Foundation Trust, University of Manchester, Manchester M13 9PL, UK. Electronic address: anna.gray@manchester.ac.uk., Schiessl I; Geoffrey Jefferson Brain Research Centre, The Manchester Academic Health Science Centre, Northern Care Alliance National Health Service Foundation Trust, University of Manchester, Manchester M13 9PL, UK; Division of Neuroscience, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK. Electronic address: i.schiessl@manchester.ac.uk., Dyer DP; Wellcome Centre for Cell-Matrix Research, Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester M13 9PT, UK; Geoffrey Jefferson Brain Research Centre, The Manchester Academic Health Science Centre, Northern Care Alliance National Health Service Foundation Trust, University of Manchester, Manchester M13 9PL, UK. Electronic address: douglas.dyer@manchester.ac.uk.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102712. Date of Electronic Publication: 2023 Nov 17.
DOI:	10.1016/j.xpro.2023.102712
Abstrakt:	The endothelial glycocalyx is an integral component of the brain vascular barrier. Visualizing its structure in vivo is essential to understand its physiological and pathophysiological mechanisms. Here, we present a surgical protocol for chronic cranial window implantation in mice, alongside the use of multiphoton microscopy tools to image the cortical vasculature. We describe steps for cranial window implantation, intravenous injection of fluorescent markers, and intravital imaging. We then detail a technique to quantify glycocalyx thickness using Imaris image analysis software. For complete details on the use and execution of this protocol, please refer to Gray et al. (2023). 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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